{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T10:25:10Z","timestamp":1788431110619,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,2,12]],"date-time":"2015-02-12T00:00:00Z","timestamp":1423699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000350","name":"Core","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000350","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ileostomy Association"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Colorectal Disease"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The aetiology of Crohn's disease\u2010related anal fistula remains obscure. Microbiological, genetic and immunological factors are thought to play a role but are not well understood. The microbiota within anal fistula tracts has never been examined using molecular techniques. The present study aimed to characterize the microbiota in the tracts of patients with Crohn's and idiopathic anal fistula.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Samples from the fistula tract and rectum of patients with Crohn's and idiopathic anal fistula were analysed using fluorescent <jats:italic>in situ<\/jats:italic> hybridization, Gram staining and scanning electron microscopy were performed to identify and quantify the bacteria present.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fifty\u2010one patients, including 20 with Crohn's anal fistula, 18 with idiopathic anal fistula and 13 with luminal Crohn's disease and no anal fistula, were recruited. Bacteria were not found in close association with the luminal surface of any of the anal fistula tracts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Anal fistula tracts generally do not harbour high levels of mucosa\u2010associated microbiota. Crohn's anal fistulas do not seem to harbour specific bacteria. Alternative explanations for the persistence of anal fistula are needed.<\/jats:p><\/jats:sec>","DOI":"10.1111\/codi.12810","type":"journal-article","created":{"date-parts":[[2014,10,30]],"date-time":"2014-10-30T14:45:14Z","timestamp":1414680314000},"page":"235-241","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["What role do bacteria play in persisting fistula formation in idiopathic and Crohn's anal fistula?"],"prefix":"10.1111","volume":"17","author":[{"given":"P. J.","family":"Tozer","sequence":"first","affiliation":[{"name":"The Fistula Research Unit St Mark's Hospital London UK"},{"name":"Antigen Presenting Research Group Imperial College London London UK"},{"name":"Northwick Park Institute for Medical Research London UK"},{"name":"School of Medicine Diabetes and Nutritional Sciences Division King's College London London UK"},{"name":"Imperial College London and St Mark's Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Rayment","sequence":"additional","affiliation":[{"name":"The Fistula Research Unit St Mark's Hospital London UK"},{"name":"School of Medicine Diabetes and Nutritional Sciences Division King's College London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. L.","family":"Hart","sequence":"additional","affiliation":[{"name":"The Fistula Research Unit St Mark's Hospital London UK"},{"name":"Antigen Presenting Research Group Imperial College London London UK"},{"name":"Imperial College London and St Mark's Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Daulatzai","sequence":"additional","affiliation":[{"name":"The Fistula Research Unit St Mark's Hospital London UK"},{"name":"Imperial College London and St Mark's Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. U.","family":"Murugananthan","sequence":"additional","affiliation":[{"name":"The Fistula Research Unit St Mark's Hospital London UK"},{"name":"Antigen Presenting Research Group Imperial College London London UK"},{"name":"Imperial College London and St Mark's Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Whelan","sequence":"additional","affiliation":[{"name":"The Fistula Research Unit St Mark's Hospital London UK"},{"name":"School of Medicine Diabetes and Nutritional Sciences Division King's College London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. K. S.","family":"Phillips","sequence":"additional","affiliation":[{"name":"The Fistula Research Unit St Mark's Hospital London UK"},{"name":"Imperial College London and St Mark's Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2007.07.152"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.1.5224.463"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.1800680802"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.2003.01561.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2006.06.021"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.21026"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.20608"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002680010250"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-005-2769-4"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.1800800853"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.1800790107"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.11.059"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.30294"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.4161\/gmic.1.3.12360"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.MIB.0000159663.62651.4f"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2005.074971"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2011.01803.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018876400528"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2005.082909"}],"container-title":["Colorectal Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcodi.12810","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/codi.12810","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T18:11:20Z","timestamp":1696356680000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/codi.12810"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,12]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,3]]}},"alternative-id":["10.1111\/codi.12810"],"URL":"https:\/\/doi.org\/10.1111\/codi.12810","archive":["Portico"],"relation":{},"ISSN":["1462-8910","1463-1318"],"issn-type":[{"value":"1462-8910","type":"print"},{"value":"1463-1318","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,12]]},"assertion":[{"value":"2014-06-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-08-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-02-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}