{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:47:19Z","timestamp":1787014039594,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,8,2]],"date-time":"2016-08-02T00:00:00Z","timestamp":1470096000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Jiangsu Province Special Program of Medical science","award":["BL2012006"],"award-info":[{"award-number":["BL2012006"]}]},{"name":"12th Five-Year Major Program of Army Research","award":["AWS12J001"],"award-info":[{"award-number":["AWS12J001"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Colorectal Disease"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      The present study was designed to investigate the function and clinical implications of stool short\u2010chain fatty acids (\n                      <jats:styled-content style=\"fixed-case\">SCFA<\/jats:styled-content>\n                      s) in patients with mixed refractory constipation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>\n                      Ascending colon specimens obtained from 30 patients with ascending colon cancer were regarded as the control group. Ascending colon specimens obtained from patients with mixed refractory constipation were regarded as the experimental group and were divided into three subgroups, according to Wexner scores [A constipation scoring system to simplify evaluation and management of constipated patients. Dis Colon Rectum 1996; 39: 681\u20135] of 16\u201320, 21\u201325 and 26\u201330, with 30 patients in each group. The stool\n                      <jats:styled-content style=\"fixed-case\">SCFA<\/jats:styled-content>\n                      s were extracted and quantitatively analysed using gas chromatography\u2010mass spectrometry (\n                      <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>\n                      \u2010\n                      <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                      ). The expression of G protein\u2010coupled receptor 43 (\n                      <jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>\n                      43) and of choline acetyltransferase (Ch\n                      <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content>\n                      ) were detected by immunofluorescence, reverse transcription\u2010polymerase chain reaction (\n                      <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\n                      \u2010\n                      <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                      ) and western blotting of colon samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The levels of acetate, propionate and butyrate were significantly lower in the experimental group than in the control group (\n                      <jats:italic>P\u00a0<\/jats:italic>\n                      &lt;\n                      <jats:italic>\u00a0<\/jats:italic>\n                      0.05). Compared with the control group, the densitometric quantification and mean density of\n                      <jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>\n                      43 and Ch\n                      <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content>\n                      proteins, and expression of\n                      <jats:italic>\n                        <jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>\n                        43\n                      <\/jats:italic>\n                      and\n                      <jats:italic>\n                        <jats:styled-content style=\"fixed-case\">CHAT<\/jats:styled-content>\n                      <\/jats:italic>\n                      genes, were significantly decreased in the patients with mixed refractory constipation (\n                      <jats:italic>P\u00a0<\/jats:italic>\n                      &lt;\n                      <jats:italic>\u00a0<\/jats:italic>\n                      0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      In the patients with mixed refractory constipation, the levels of stool\n                      <jats:styled-content style=\"fixed-case\">SCFA<\/jats:styled-content>\n                      s, including acetate, propionate and butyrate, as well as the levels of\n                      <jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>\n                      43 and Ch\n                      <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content>\n                      expressed in the colon, which were all negatively correlated with the Wexner score, were decreased and may be associated with the pathogenesis of mixed refractory constipation.\n                    <\/jats:p>\n                  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