{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T15:11:04Z","timestamp":1788189064439,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,12,2]],"date-time":"2016-12-02T00:00:00Z","timestamp":1480636800000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003958","name":"Stichting voor de Technische Wetenschappen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003958","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003195","name":"Ministerie van Economische Zaken","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003195","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Colorectal Disease"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Nodal status is the most important prognostic factor in colorectal cancer (<jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content>). Small occult metastases may remain undetected on conventional histopathological examination, potentially resulting in undertreatment. <jats:italic>Ex vivo<\/jats:italic> sentinel lymph node mapping (<jats:styled-content style=\"fixed-case\">SLNM<\/jats:styled-content>) can be used to improve the accuracy of nodal staging, but the currently used tracers suffer from drawbacks, which hamper implementation of the technique in routine clinical practice. Magnetic tracers are the optimal size for sentinel lymph node (<jats:styled-content style=\"fixed-case\">SLN<\/jats:styled-content>) retention and allow objective quantitative selection of <jats:styled-content style=\"fixed-case\">SLN<\/jats:styled-content>s; they therefore have great potential for <jats:styled-content style=\"fixed-case\">SLNM<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content>. The study evaluates the feasibility of <jats:italic>ex\u00a0vivo<\/jats:italic> magnetic <jats:styled-content style=\"fixed-case\">SLNM<\/jats:styled-content> and compares the performance of this technique with blue dye <jats:styled-content style=\"fixed-case\">SLNM<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Twenty\u2010eight <jats:italic>ex\u00a0vivo <\/jats:italic><jats:styled-content style=\"fixed-case\">SLNM<\/jats:styled-content> procedures were performed in 27 histological node\u2010negative patients with <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> using a magnetic tracer and blue dye. A magnetometer was used to select magnetic <jats:styled-content style=\"fixed-case\">SLN<\/jats:styled-content>s after formalin fixation of the <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> specimen. Both magnetic and blue <jats:styled-content style=\"fixed-case\">SLN<\/jats:styled-content>s were subjected to serial sectioning and\u00a0immunohistochemical staining to reveal occult metastases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At least one <jats:styled-content style=\"fixed-case\">SLN<\/jats:styled-content> was successfully identified in 27\/28 (96%) and 25\/28 (89%) of the cases with the magnetic technique and blue dye. Isolated tumour cells were detected in 10 patients. This was predicted with 100% sensitivity and accuracy using the magnetic technique, and with 91% sensitivity and 96% accuracy using the blue dye technique.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study demonstrates that <jats:italic>ex\u00a0vivo<\/jats:italic> magnetic <jats:styled-content style=\"fixed-case\">SLNM<\/jats:styled-content> is a feasible technique for use in routine clinical practice, improving nodal staging accuracy of <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/codi.13395","type":"journal-article","created":{"date-parts":[[2016,5,24]],"date-time":"2016-05-24T20:21:43Z","timestamp":1464121303000},"page":"1147-1153","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["<i>Ex vivo<\/i> sentinel lymph node mapping in colorectal cancer using a magnetic nanoparticle tracer to improve staging accuracy: a pilot study"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4401-5768","authenticated-orcid":false,"given":"J. 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