{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T14:55:58Z","timestamp":1783781758422,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,6]],"date-time":"2025-07-06T00:00:00Z","timestamp":1751760000000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82070685"],"award-info":[{"award-number":["82070685"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Colorectal Disease"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Assessing tumour response following neoadjuvant chemoradiotherapy (nCRT) in patients with locally advanced rectal cancer is essential for selecting patients for the watch\u2010and\u2010wait procedure. The accuracy of clinical complete response (cCR) for predicting pathological complete response (pCR) is inadequate. The aim of this study was to assess the ability of transanal multipoint full\u2010layer puncture biopsy (TMFP) to predict pCR following nCRT for rectal cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>This nonrandomized prospective multicentre trial enrolled 113 patients from four centres between April 2020 and June 2023. Of these, 63 were assigned to the <jats:italic>in vitro<\/jats:italic> TMFP group and 50 to the in\u00a0vivo TMFP group. The primary outcome measured was the predictive accuracy of TMFP for pCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All patients underwent total mesorectal excision. TMFP showed significantly higher accuracy for predicting pCR than cCR (87.6% vs. 68.1%, <jats:italic>p<\/jats:italic>\u2009=\u20090.016). The positive predictive value was higher for in\u00a0vivo than for in\u00a0vitro TMFP (90.0% vs. 64.5%, <jats:italic>p<\/jats:italic>\u2009=\u20090.018), and the predictive accuracy did not differ significantly between the two groups (94.0% vs. 82.5%, <jats:italic>p<\/jats:italic>\u2009=\u20090.066). In cCR patients, although with no significant difference between in\u00a0vivo and in\u00a0vitro TMFP regarding positive predictive value (95.0% vs. 86.7%, <jats:italic>p<\/jats:italic>\u2009=\u20090.794) and accuracy (96.0% vs. 92.9%, <jats:italic>p<\/jats:italic>\u2009=\u20091.000), the predictive accuracy of in\u00a0vivo TMFP was as high as 96%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>TMFP is safe and feasible for assessing the response of rectal cancer to nCRT. TMFP showed high sensitivity and accuracy for predicting pCR and could be a useful supplement to the current cCR standard.<\/jats:p><\/jats:sec>","DOI":"10.1111\/codi.70148","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:22:49Z","timestamp":1751847769000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Value of transanal multipoint full\u2010layer puncture biopsy for determining the response of rectal cancer to neoadjuvant therapy: A prospective multicentre cohort study"],"prefix":"10.1111","volume":"27","author":[{"given":"Jia Gang","family":"Han","sequence":"first","affiliation":[{"name":"Department of General Surgery, Beijing Chaoyang Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li Ting","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Beijing Friendship Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping Dian","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of General Surgery Qilu Hospital of Shandong University  Jinan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi Wei","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Beijing Chaoyang Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong Qing","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of General Surgery Zhongnan Hospital of Wuhan University  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Epidemiology Laboratory, Beijing Institute of Respiratory Medicine, Beijing Chaoyang Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qun","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of General Surgery Zhongnan Hospital of Wuhan University  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of General Surgery Qilu Hospital of Shandong University  Jinan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong Wei","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Beijing Friendship Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Beijing Chaoyang Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,6]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)61649-9"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2022.0051"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(20)30555-6"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijsu.2018.06.031"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/DCR.0b013e31829ef472"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02239429"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(18)30301-7"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)31078-X"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2018.5896"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/DCR.0000000000002197"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/DCR.0b013e3182a25c4e"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdy161"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-015-1632-z"},{"key":"e_1_2_13_15_1","first-page":"1","article-title":"Consensus on the watch and wait policy in rectal cancer patients after neoadjuvant treatment (2020 version)","volume":"23","author":"Chinese Watch & Wait Database Research Cooperation Group(CWWD); 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