{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T10:13:47Z","timestamp":1753438427602,"version":"3.37.3"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,2,4]],"date-time":"2014-02-04T00:00:00Z","timestamp":1391472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003035","name":"Aase and Ejnar Danielsens Fond","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003035","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007460","name":"Arvid Nilssons Fond","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007460","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004331","name":"Snedkermester Sophus Jacobsen og hustru Astrid Jacobsens Fond","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004331","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Physio Funct Imaging"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Inflammation, and specifically adipose tissue (<jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content>) inflammation, is part of the pathophysiology of obesity and <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010associated lipodystrophy. Local <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content> protein assessment methods are limited, and <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content> inflammation studies have therefore primarily examined inflammatory gene expression. We therefore investigated the utility of microdialysis to study <jats:italic>in situ <\/jats:italic><jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content> interstitial inflammatory protein levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Abdominal subcutaneous <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content> microdialysis was performed in six healthy men, six <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected men with lipodystrophy and six without lipodystrophy using the internal references <jats:sup>51<\/jats:sup>Cr\u2010<jats:styled-content style=\"fixed-case\">EDTA<\/jats:styled-content> and <jats:sup>125<\/jats:sup>I\u2010human serum albumin. We measured 41 inflammatory proteins in microdialysis samples by <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>uminex technology, as well as systemic levels in 14 subjects. Furthermore, <jats:italic>in vitro<\/jats:italic> studies of the internal reference technique for microdialysis recovery of inflammatory proteins were made.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We detected <jats:italic>in situ <\/jats:italic><jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content> interstitial levels of 14 inflammatory proteins by microdialysis, while the 27 other inflammatory proteins assessed were only detected sporadically. Initial levels of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108 were undetectable. Insertion trauma affected <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b1, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108, monocyte chemotactic factor (<jats:styled-content style=\"fixed-case\">MCP<\/jats:styled-content>)\u20101, <jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content>\u201010, G\u2010<jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>, growth\u2010related oncogene (<jats:styled-content style=\"fixed-case\">GRO<\/jats:styled-content>), macrophage\u2010derived chemokine (<jats:styled-content style=\"fixed-case\">MDC<\/jats:styled-content>) and macrophage inflammatory protein (<jats:styled-content style=\"fixed-case\">MIP<\/jats:styled-content>)\u20101\u03b2 levels, while fibroblast growth factor (<jats:styled-content style=\"fixed-case\">FGF<\/jats:styled-content>)\u20102 was not affected. Systemic and <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content> interstitial levels were poorly correlated. The microdialysis recovery of smaller proteins was higher than for larger, and the internal references improved microdialysis by accounting for variation in perfusion across the membrane.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Interstitial inflammatory proteins can be sampled <jats:italic>in situ<\/jats:italic> using microdialysis. Use of internal references improves the microdialysis technique. However, insertion trauma hampers the use of microdialysis to study <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content> inflammatory levels, except for <jats:styled-content style=\"fixed-case\">FGF<\/jats:styled-content>\u20102. Still, microdialysis gives unique insight to <jats:italic>in situ <\/jats:italic><jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content> interstitial concentrations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpf.12134","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T01:51:01Z","timestamp":1391565061000},"page":"110-119","source":"Crossref","is-referenced-by-count":8,"title":["Assessment of <i>in situ<\/i> adipose tissue inflammation by microdialysis"],"prefix":"10.1111","volume":"35","author":[{"given":"Anne","family":"Langkilde","sequence":"first","affiliation":[{"name":"Clinical Research Centre Copenhagen University Hospital Hvidovre Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ove","family":"Andersen","sequence":"additional","affiliation":[{"name":"Clinical Research Centre Copenhagen University Hospital Hvidovre Denmark"},{"name":"Department of Infectious Diseases Copenhagen University Hospital Hvidovre Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens H.","family":"Henriksen","sequence":"additional","affiliation":[{"name":"Department of Clinical Physiology and Nuclear Medicine Copenhagen University Hospital Hvidovre Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henning","family":"Langberg","sequence":"additional","affiliation":[{"name":"CopenRehab Section of Social Medicine Department of Public Health Faculty of Health Sciences University of Copenhagen Copenhagen Denmark"},{"name":"Institute of Sports Medicine Copenhagen Copenhagen University Hospital Bispebjerg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janne","family":"Petersen","sequence":"additional","affiliation":[{"name":"Clinical Research Centre Copenhagen University Hospital Hvidovre 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