{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T07:47:48Z","timestamp":1696405668413},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,5,27]],"date-time":"2015-05-27T00:00:00Z","timestamp":1432684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The John and Birthe Meyer Foundation"},{"name":"Savvaerksejer Jeppe Juhl and Wife Ovita Juhl's Memorial Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Physio Funct Imaging"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Patients with advanced cirrhosis have abnormal sodium homoeostasis. The study was undertaken to quantify the sodium transport across the plasma membrane of red blood cells (<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>) in patients with cirrhosis. <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> efflux and influx of sodium were studied <jats:italic>in vitro<\/jats:italic> with tracer <jats:sup>22<\/jats:sup>Na<jats:sup>+<\/jats:sup> according to linear kinetics in 24 patients with cirrhosis and 14 healthy controls. The sodium efflux was modified by ouabain (O), furosemide (F) and a combination of O and F (O\u00a0+\u00a0F). <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> sodium was significantly decreased (4\u00b76 versus control 6\u00b73\u00a0mmol\u00a0l<jats:sup>\u22121<\/jats:sup>, <jats:italic>P<\/jats:italic>&lt;0\u00b7001) and directly related to serum sodium (<jats:italic>r<\/jats:italic>\u00a0=\u00a00\u00b757, <jats:italic>P<\/jats:italic>&lt;0\u00b705). The <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> fractional sodium efflux was higher in patients with cirrhosis (+46%, <jats:italic>P<\/jats:italic>&lt;0\u00b701) compared to controls. Inhibition in both high (145\u00a0mmol\u00a0l<jats:sup>\u22121<\/jats:sup>)\u2010 and low (120\u00a0mmol\u00a0l<jats:sup>\u22121<\/jats:sup>)\u2010sodium buffers showed that the F\u2010insensitive sodium efflux was twice as high in cirrhosis as in controls (<jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b703\u20130\u00b7007), especially the O\u2010sensitive, F\u2010insensitive efflux was increased (+ 225%, <jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b701\u20130\u00b7006). Fractional F\u2010sensitive transport was normal in cirrhosis. <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> sodium influx was largely normal in cirrhosis. In conclusion, <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> sodium content is reduced in patients with cirrhosis with a direct relation to serum sodium. Increased <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> sodium efflux is especially related to ouabain\u2010sensitive, furosemide\u2010insensitive transport and thus most likely due to upregulated activity of the sodium\u2013potassium pump. The study gives no evidence to an altered intracellular\/extracellular sodium ratio or to a reduced fractional furosemide\u2010sensitive sodium transport in cirrhosis.<\/jats:p>","DOI":"10.1111\/cpf.12238","type":"journal-article","created":{"date-parts":[[2015,5,28]],"date-time":"2015-05-28T12:45:09Z","timestamp":1432817109000},"page":"359-367","source":"Crossref","is-referenced-by-count":0,"title":["Red blood cell sodium transport in patients with cirrhosis"],"prefix":"10.1111","volume":"36","author":[{"given":"Ulrik L\u00fctken","family":"Henriksen","sequence":"first","affiliation":[{"name":"Department of Clinical Physiology 239 Center for Functional and Diagnostic Imaging and Research University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marianne","family":"Kiszka\u2010Kanowitz","sequence":"additional","affiliation":[{"name":"Gastrounit Medical Division Hvidovre Hospital Faculty of Medicine and Health Sciences University of 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