{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T06:09:57Z","timestamp":1771567797396,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,3,19]],"date-time":"2013-03-19T00:00:00Z","timestamp":1363651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81060091"],"award-info":[{"award-number":["81060091"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Medical Elitist Project of Jiangsu Province","award":["RC2011140"],"award-info":[{"award-number":["RC2011140"]}]},{"name":"Nature Science Foundation of Jiangsu Province","award":["BK2009346"],"award-info":[{"award-number":["BK2009346"]}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2013,4]]},"abstract":"Abstract<\/jats:title>Objectives<\/jats:title>Stem cells from the dental apical papilla (SCAP<\/jats:styled-content>s) can be induced to differentiate along both osteoblast and odontoblast lineages. However, little knowledge is available concerning their differentiation efficiency in osteogenic media containing additional KH<\/jats:styled-content>2<\/jats:sub>PO<\/jats:styled-content>4<\/jats:sub>.<\/jats:p><\/jats:sec>Materials and methods<\/jats:title>Stem cells from the dental apical papilla were isolated from apical papillae of immature third molars and treated with two kinds of mineralization\u2010inducing media, MM<\/jats:styled-content>1 and MM<\/jats:styled-content>2, differing in KH<\/jats:styled-content>2<\/jats:sub>PO<\/jats:styled-content>4<\/jats:sub> concentration. Proliferation and osteo\/odontogenic differentiation capacity of MM<\/jats:styled-content>1\/MM<\/jats:styled-content>2\u2010treated SCAP<\/jats:styled-content>s were investigated and compared both in vitro<\/jats:italic> and in vivo<\/jats:italic>.<\/jats:p><\/jats:sec>Results<\/jats:title>Cell counting and flow cytometry demonstrated that MM<\/jats:styled-content>2 containing 1.8\u00a0mm<\/jats:sc> additional KH<\/jats:styled-content>2<\/jats:sub>PO<\/jats:styled-content>4<\/jats:sub> significantly enhanced proliferative potential of SCAP<\/jats:styled-content>s, compared to MM<\/jats:styled-content>1. Osteo\/odontogenic capacity of SCAP<\/jats:styled-content>s was much better in MM<\/jats:styled-content>2 medium than in MM<\/jats:styled-content>1, as indicated by elevated alkaline phosphatase activity, increased calcium deposition and upregulated expression of osteo\/odontoblast\u2010specific genes\/proteins (for example, runt\u2010related transcription factor 2, osterix, osteocalcin, dentin sialoprotein, and dentin sialophosphoprotein). In vivo<\/jats:italic> transplantation findings proved that SCAP<\/jats:styled-content>s in MM<\/jats:styled-content>2 group generated more mineralized tissues, and presented higher expression of osteo\/odontoblast\u2010specific proteins (osteocalcin and dentin sialoprotein) than those in the MM<\/jats:styled-content>1 group.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Mineralization\u2010inducing media supplemented with 1.8\u00a0mm<\/jats:sc> additional KH<\/jats:styled-content>2<\/jats:sub>PO<\/jats:styled-content>4<\/jats:sub> significantly enhanced cell proliferation and improved differentiation capacity of SCAP<\/jats:styled-content>s along osteo\/odontogenic cell lineages, compared to counterparts lacking additional KH<\/jats:styled-content>2<\/jats:sub>PO<\/jats:styled-content>4<\/jats:sub>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12016","type":"journal-article","created":{"date-parts":[[2013,3,19]],"date-time":"2013-03-19T13:24:57Z","timestamp":1363699497000},"page":"214-222","source":"Crossref","is-referenced-by-count":31,"title":["Proliferation and osteo\/odontoblastic differentiation of stem cells from dental apical papilla in mineralization\u2010inducing medium containing additional KH<\/scp>2<\/sub>PO<\/scp>4<\/sub>"],"prefix":"10.1111","volume":"46","author":[{"given":"L.","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Stomatology Nanjing Medical University Nanjing 210029 Jiangsu China"},{"name":"College of Stomatology Guangxi Medical University Nanning 530021 Guangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Yan","sequence":"additional","affiliation":[{"name":"Institute of Stomatology Nanjing Medical University Nanjing 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