{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:34:15Z","timestamp":1792629255958,"version":"4.5.0"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,3,19]],"date-time":"2013-03-19T00:00:00Z","timestamp":1363651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      Small aromatic compounds like flavonoids can intercalate with\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      molecules bringing about conformational changes leading to reduced replication and transcription. Here, we have examined one dietary flavonoid, quercetin (found in many fruit and vegetables), for possible anti\u2010cancer effects, on HeLa cells originally derived from a case of human cervical cancer.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and methods<\/jats:title>\n                    <jats:p>\n                      By circular dichroism spectroscopy we tested whether quercetin effectively interacted with\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      to bring about conformational changes that would strongly inhibit proliferation and migration of the HeLa cells. Cytotoxic effects of quercetin on cancer\/normal cells, if any, were determined by\n                      <jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content>\n                      assay and such depolarization of mitochondrial membrane potential, as a consequence of quercetin treatment, and accumulation of reactive oxygen species (\n                      <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>\n                      ) also were studied, by\n                      <jats:styled-content style=\"fixed-case\">FACS<\/jats:styled-content>\n                      analysis and expression profiles of different anti\u2010 and pro\u2010apoptotic genes and their products were determined.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Quercetin intercalated with calf thymus cell\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      and HeLa cell\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      and inhibition of anti\u2010apoptotic\n                      <jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content>\n                      and Bcl\u20102 expression were observed. Levels of mitochondrial cytochrome\u2010c were elevated and depolarization of mitochondrial membrane potential occurred with increase of\n                      <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>\n                      ; upregulation of expression of p53 and caspase\u20103 activity were also noted. These alterations in signalling proteins and externalization of phosphotidyl serine residues were involved with initiation of apoptosis. Reduced\n                      <jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content>\n                      expression suggested reduction in cell proliferation and metastasis potential, with arrest of the cell cycle at G2\/M.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Quercetin would have potential for use in cervical cancer chemotherapy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cpr.12017","type":"journal-article","created":{"date-parts":[[2013,3,19]],"date-time":"2013-03-19T09:24:57Z","timestamp":1363685097000},"page":"153-163","source":"Crossref","is-referenced-by-count":160,"title":["Quercetin induces cytochrome\u2010c release and\n                    <scp>ROS<\/scp>\n                    accumulation to promote apoptosis and arrest the cell cycle in G2\/M, in cervical carcinoma: signal cascade and drug\u2010DNA interaction"],"prefix":"10.1111","volume":"46","author":[{"given":"K.","family":"Bishayee","sequence":"first","affiliation":[{"name":"Department of Zoology Cytogenetics and Molecular Biology Laboratory University of Kalyani Kalyani 741235 West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Department of Zoology Cytogenetics and Molecular Biology Laboratory University of Kalyani Kalyani 741235 West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Department of Zoology Cytogenetics and Molecular Biology Laboratory University of Kalyani Kalyani 741235 West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Sadhukhan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics University of Kalyani Kalyani 741235 West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Mondal","sequence":"additional","affiliation":[{"name":"Department of Zoology Cytogenetics and Molecular Biology Laboratory University of Kalyani Kalyani 741235 West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. R.","family":"Khuda\u2010Bukhsh","sequence":"additional","affiliation":[{"name":"Department of Zoology Cytogenetics and Molecular Biology Laboratory University of Kalyani Kalyani 741235 West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,3,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1207\/s15327914nc5001_1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf9811065"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwm172"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6602364"},{"key":"e_1_2_7_6_1","first-page":"60","article-title":"Effects of quercetin on proliferation, apoptosis, adhesion and migration, and invasion of HeLa cells","volume":"30","author":"Zhang W","year":"2009","journal-title":"Eur. J. Gynaecol. 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