{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:52:23Z","timestamp":1788871943349,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,5,22]],"date-time":"2013-05-22T00:00:00Z","timestamp":1369180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31070727"],"award-info":[{"award-number":["31070727"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["30670889"],"award-info":[{"award-number":["30670889"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81273170"],"award-info":[{"award-number":["81273170"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Science Technology Major Special Project on Major New Drug Innovation","award":["2010ZX09401"],"award-info":[{"award-number":["2010ZX09401"]}]},{"name":"National \u201cTwelfth Five-Year\u201d Plan for Science and Technology Support","award":["2012BAI37B03"],"award-info":[{"award-number":["2012BAI37B03"]}]},{"name":"Major State Basic Research Program-973 of China","award":["2011CB503806"],"award-info":[{"award-number":["2011CB503806"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      Human CAP10\u2010like protein 46 kDa (\n                      <jats:styled-content style=\"fixed-case\">hCLP<\/jats:styled-content>\n                      46), also known as Poglut1, has been shown to be an essential regulator of Notch signalling.\n                      <jats:styled-content style=\"fixed-case\">hCLP<\/jats:styled-content>\n                      46 is overexpressed in primary acute myelogenous leukaemia, T\u2010acute lymphoblastic leukaemia samples and other leukaemia cell lines. However, effects of\n                      <jats:styled-content style=\"fixed-case\">hCLP<\/jats:styled-content>\n                      46 overexpression, up to now, have remained unknown.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>\n                      In this study, we established stable 293TRex cell lines inducibly overexpressing\n                      <jats:styled-content style=\"fixed-case\">hCLP<\/jats:styled-content>\n                      46, and knocked down\n                      <jats:styled-content style=\"fixed-case\">hCLP<\/jats:styled-content>\n                      6 with a specific small interfering RNA to explore function of the protein in Notch signalling and cell proliferation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:styled-content style=\"fixed-case\">hCLP<\/jats:styled-content>\n                      46 overexpression enhanced Notch1 activation in 293Trex cells in a ligand\u2010dependent manner, with increased Notch signalling enhancing Hes1 expression. We further verified that overexpression of\n                      <jats:styled-content style=\"fixed-case\">hCLP<\/jats:styled-content>\n                      46 inhibited proliferation of 293TRexs and was correlated with increases in cyclin dependent kinase inhibitors p21 and p27, whereas reduced\n                      <jats:styled-content style=\"fixed-case\">hCLP<\/jats:styled-content>\n                      46 expression moderately increased cell proliferation. In addition, p21 and p27 protein levels were higher when Notch signalling was activated by\n                      <jats:styled-content style=\"fixed-case\">EDTA<\/jats:styled-content>\n                      treatment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Taken together,\n                      <jats:styled-content style=\"fixed-case\">hCLP<\/jats:styled-content>\n                      46 enhanced Notch activation and inhibited 293TRex cell proliferation through CDKI signalling.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cpr.12037","type":"journal-article","created":{"date-parts":[[2013,5,22]],"date-time":"2013-05-22T01:03:45Z","timestamp":1369184625000},"page":"254-262","source":"Crossref","is-referenced-by-count":21,"title":["Overexpression of\n                    <scp>hCLP<\/scp>\n                    46 enhances Notch activation and regulates cell proliferation in a cell type\u2010dependent manner"],"prefix":"10.1111","volume":"46","author":[{"given":"Q.","family":"Chu","sequence":"first","affiliation":[{"name":"College of Life Sciences University of Chinese Academy of Sciences Beijing 100049 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Sciences University of Chinese Academy of Sciences Beijing 100049 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences University of Chinese Academy of Sciences Beijing 100049 China"},{"name":"School of Medical Sciences Edith Cowan University Perth WA 6027 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