{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:55:13Z","timestamp":1787061313269,"version":"build-2736575974"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,1,22]],"date-time":"2014-01-22T00:00:00Z","timestamp":1390348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2014,2]]},"abstract":"Abstract<\/jats:title>\n \n Objectives<\/jats:title>\n \n Terminal restriction fragment (\n TRF<\/jats:styled-content>\n ) analysis of human telomeres was used to calibrate flow\u2010fluorescence\n in situ<\/jats:italic>\n hybridization (\n FF<\/jats:styled-content>\n ) measures of telomere lengths to expand the range of measures and increase power of resolution of our previously published protocol.\n TRF<\/jats:styled-content>\n data used as the gold standard should be obtained by electrophoresis with suitable resolution applied to appropriately isolated genomic\n DNA<\/jats:styled-content>\n . When we considered\n TRF<\/jats:styled-content>\n attained by correct methods, we found our method to be insufficiently accurate, thus we have reviewed our previously published\n FF<\/jats:styled-content>\n protocol to obtain the best coefficient of determination (\n r<\/jats:italic>\n 2<\/jats:sup>\n ) between our experimental results and valid\n TRF<\/jats:styled-content>\n lengths.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and methods<\/jats:title>\n \n Using human telomere\u2010specific\n PNA<\/jats:styled-content>\n probe, Cy5\u2010OO\u2010(CCCTAA)\n 3<\/jats:sub>\n , we measured telomere lengths of continuous cell line and of peripheral blood lymphocytes by\n FF<\/jats:styled-content>\n . We modified hybridization, stringency, negative control handling, stoichiometric\n DNA<\/jats:styled-content>\n staining and telomere fluorescence assessment of the protocol.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n We realized a procedure with increased power of resolution, improved\n TRF<\/jats:styled-content>\n versus\n FF<\/jats:styled-content>\n r<\/jats:italic>\n 2<\/jats:sup>\n values that allowed simultaneous analysis of\n DNA<\/jats:styled-content>\n and telomere duplication. Notwithstanding multiple steps in formamide sampling, recovery was satisfactory.\n <\/jats:p>\n <\/jats:sec>\n \n Discussion<\/jats:title>\n \n The reviewed\n FF<\/jats:styled-content>\n protocol appeared at least as suitable as the\n TRF<\/jats:styled-content>\n method. Measures obtained by\n TRF<\/jats:styled-content>\n can be affected by chromosome end variability,\n DNA<\/jats:styled-content>\n fragmentation, incomplete digestion and unsuitable electrophoresis. In contrast, the\n FF<\/jats:styled-content>\n technique analyses telomeric sequences confined to preserved nuclei thus overcome most previous limitations. As yet, however, the\n FF<\/jats:styled-content>\n telomere measure cannot be performed together with immunophenotyping and\/or generation study by the dye dilution method.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/cpr.12086","type":"journal-article","created":{"date-parts":[[2014,1,22]],"date-time":"2014-01-22T11:41:41Z","timestamp":1390390901000},"page":"20-27","source":"Crossref","is-referenced-by-count":6,"title":["Correlation between terminal restriction fragments and flow\u2010\n FISH<\/scp>\n measures in samples over wide range telomere lengths"],"prefix":"10.1111","volume":"47","author":[{"given":"M.","family":"Carbonari","sequence":"first","affiliation":[{"name":"Dipartimento di Medicina Clinica Universit\u00e0 di Roma \u201cLa Sapienza\u201d 00185 Roma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Tedesco","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Clinica Universit\u00e0 di Roma \u201cLa Sapienza\u201d 00185 Roma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Fiorilli","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Clinica Universit\u00e0 di Roma \u201cLa Sapienza\u201d 00185 Roma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,1,22]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.16.3651"},{"key":"e_1_2_5_3_1","first-page":"1039","article-title":"Comparison of different protocols for telomere length estimation by combination of quantitative fluorescence in situ hybridization (Q\u2010FISH) and flow cytometry in human cancer cell lines","volume":"25","author":"Derradji H","year":"2005","journal-title":"Anticancer Res."},{"key":"e_1_2_5_4_1","volume-title":"Molecular Cloning a Laboratory Manual","author":"Sambrook J","year":"2001"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-005-0557-2"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21675"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2184.2010.00704.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.10064"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1984.tb01890.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/904767"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10577-005-0998-1"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri890"},{"key":"e_1_2_5_13_1","first-page":"51","article-title":"Measurement of DNA repeat sequences by flow cytometry","volume":"9","author":"Dragowska V","year":"1998","journal-title":"Cytometry"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.10053"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201040862"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2004.06.003"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2008.06.001"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/990141"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0598-32"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.10163"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20181"}],"container-title":["Cell Proliferation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcpr.12086","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cpr.12086","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T02:52:35Z","timestamp":1696301555000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cpr.12086"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,22]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,2]]}},"alternative-id":["10.1111\/cpr.12086"],"URL":"https:\/\/doi.org\/10.1111\/cpr.12086","archive":["Portico"],"relation":{},"ISSN":["0960-7722","1365-2184"],"issn-type":[{"value":"0960-7722","type":"print"},{"value":"1365-2184","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,22]]}}}