{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T18:49:46Z","timestamp":1790275786791,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,1,27]],"date-time":"2014-01-27T00:00:00Z","timestamp":1390780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      To evaluate anti\u2010proliferative as well as apoptotic activities of compounds identified in chloroform extract of\n                      <jats:italic>Juglans regia<\/jats:italic>\n                      leaves, on human breast and oral cancer cell lines (\n                      <jats:styled-content style=\"fixed-case\">MCF<\/jats:styled-content>\n                      \u20107 and\n                      <jats:styled-content style=\"fixed-case\">BHY<\/jats:styled-content>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>\n                      Column chromatography,\n                      <jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content>\n                      assay, flowcytometry and western blotting have all been used in the study.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Bioassay\u2010guided fractionation of chloroform extract of\n                      <jats:italic>J.\u00a0regia<\/jats:italic>\n                      afforded isolation of 5\u2010hydroxy\u20103,7,4\u2032\u2010trimethoxyflavone [\n                      <jats:bold>1<\/jats:bold>\n                      ], lupeol [\n                      <jats:bold>2<\/jats:bold>\n                      ], daucosterol [\n                      <jats:bold>3<\/jats:bold>\n                      ], 4\u2010hydroxy\u2010\u03b1\n                      <jats:italic>\u2010<\/jats:italic>\n                      tetralone [\n                      <jats:bold>4<\/jats:bold>\n                      ], \u03b2\u2010sitosterol [\n                      <jats:bold>5<\/jats:bold>\n                      ], 5,7\u2010 dihydroxy\u20103,4\u2032\u2010dimethoxyflavone [\n                      <jats:bold>6<\/jats:bold>\n                      ] and regiolone [\n                      <jats:bold>7<\/jats:bold>\n                      ]. Structures of the compounds were established on the basis of spectroscopic analyses [Nuclear magnetic resonance (NMR) and mass]. All compounds inhibited proliferation of\n                      <jats:styled-content style=\"fixed-case\">MCF<\/jats:styled-content>\n                      \u20107 (human breast adenocarcinoma) and\n                      <jats:styled-content style=\"fixed-case\">BHY<\/jats:styled-content>\n                      (human oral squamous carcinoma) cells in a concentration\u2010dependent manner. Compounds\n                      <jats:bold>6<\/jats:bold>\n                      and\n                      <jats:bold>7<\/jats:bold>\n                      had potent cytotoxic effects on both\n                      <jats:styled-content style=\"fixed-case\">MCF<\/jats:styled-content>\n                      \u20107 and\n                      <jats:styled-content style=\"fixed-case\">BHY<\/jats:styled-content>\n                      cells (\n                      <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content>\n                      <jats:sub>50<\/jats:sub>\n                      21\u201351\u00a0\u03bc\n                      <jats:sc>m<\/jats:sc>\n                      ), yet were not toxic to normal cells.\n                      <jats:styled-content style=\"fixed-case\">MCF<\/jats:styled-content>\n                      \u20107 growth inhibition was attributed to apoptosis; population of apoptotic cells increased from 1.12% in controls to 5.64 and 8.1% after 48\u2010h treatment with compounds\n                      <jats:bold>6<\/jats:bold>\n                      and\n                      <jats:bold>7<\/jats:bold>\n                      , indicating their potential at inducing early and late apoptosis. The caspase cascade was not activated, as indicated by only insignificant cleavage of caspase\u20103.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Our results suggest that compounds\n                      <jats:bold>6<\/jats:bold>\n                      and\n                      <jats:bold>7<\/jats:bold>\n                      can induce apoptosis in\n                      <jats:styled-content style=\"fixed-case\">MCF<\/jats:styled-content>\n                      \u20107 cells through the caspase\u20103 independent pathway.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cpr.12090","type":"journal-article","created":{"date-parts":[[2014,1,27]],"date-time":"2014-01-27T12:47:27Z","timestamp":1390826847000},"page":"172-179","source":"Crossref","is-referenced-by-count":36,"title":["Anti\u2010proliferative and apoptotic activities of constituents of chloroform extract of\n                    <i>Juglans regia<\/i>\n                    leaves"],"prefix":"10.1111","volume":"47","author":[{"given":"M.","family":"Salimi","sequence":"first","affiliation":[{"name":"Department of Physiology and Pharmacology Pasteur Institute of Iran Tehran 3159915111 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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