{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T17:33:17Z","timestamp":1768325597887,"version":"3.49.0"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T00:00:00Z","timestamp":1392163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objectives<\/jats:italic>: Increasing evidence has suggested the close relationship between micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) dysregulation and the carcinogenesis of Ewing's sarcoma (<jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content>), among of which miR\u2010125b has been reported to be decreased in <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content> tissues recently. Strikingly, ectopic expression of miR\u2010125b could suppress cell proliferation of <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content> cell line A673, suggesting the tumor suppressor role of miR\u2010125b in <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content>. However, the other accurate mechanistic functions and relative molecule mechanisms are largely unknown.<\/jats:p><jats:p><jats:italic>Materials and Methods<\/jats:italic>: Herein, we completed a series of experiments to investigate the role of miR\u2010125b in Ewing's sarcoma. We restored the expression of miR\u2010125b in <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content> cell line A673 through transfection with miR\u2010125b mimics. To further understand the role of miR\u2010125b in <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content>, we detected the effects of miR\u2010125b on the cell proliferation, migration and invasion, cell cycle as well as cell apoptosis.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>: We found that restored expression of miR\u2010125b in ES cell line A673 inhibited cell proliferation, migration and invasion, arrested cell cycle progression, and induced cell apoptosis. Moreover, bioinformatic prediction suggested the oncogene, phosphoinositide\u20103\u2010kinase catalytic subunit delta (<jats:italic>PIK3CD<\/jats:italic>), was a target gene of miR\u2010125b in ES cells. Further quantitative RT\u2010PCR and western blot assays identified over\u2010expression of miR\u2010125b suppressed the expression of <jats:italic>PIK3CD <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and protein. <jats:italic>PIK3CD<\/jats:italic> participates in regulating the <jats:italic>PI3K<\/jats:italic> signaling pathway, which has been reported to play an important role in the development of ES. Suppression of <jats:italic>PIK3CD<\/jats:italic> down\u2010regulated the expression of phospho\u2010<jats:italic>AKT<\/jats:italic> and phospho\u2010<jats:italic><jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content><\/jats:italic> proteins and inhibited the biologic progression of A673 cells.<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>: Collectively, these data suggest that miR\u2010125b functions as a tumor suppressor by targeting the PI3K\/Akt\/<jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content> signaling pathway, and may provide potential therapy strategy for <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content> patients by targeting mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression.<\/jats:p>","DOI":"10.1111\/cpr.12093","type":"journal-article","created":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T11:59:33Z","timestamp":1392206373000},"page":"152-160","source":"Crossref","is-referenced-by-count":84,"title":["MiR\u2010125b inhibits cell biological progression of Ewing's sarcoma by suppressing the PI3K\/Akt signalling pathway"],"prefix":"10.1111","volume":"47","author":[{"given":"J.","family":"Li","sequence":"first","affiliation":[{"name":"Department of Orthopaedics The Second Hospital of Anhui Medical University Hefei Anhui 230601 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"You","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The First Hospital of Anhui Medical University Hefei Anhui 230601 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Jing","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The Second Hospital of Anhui Medical University Hefei Anhui 230601 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,2,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jjco\/hyl142"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2013.00057"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(12)70073-6"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9523"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023592"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2011.197"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2011.497"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035435"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/CMR.0b013e32833e32a1"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-012-1288-x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000343320"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1038\/ncb2613","article-title":"The functions of microRNAs in for review only pluripotency and reprogramming","volume":"14","author":"Leonardo TR","journal-title":"Nat. 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