{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:43:07Z","timestamp":1777444987944,"version":"3.51.4"},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,4,8]],"date-time":"2015-04-08T00:00:00Z","timestamp":1428451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2015,6]]},"abstract":"Abstract<\/jats:title>\n \n Objectives<\/jats:title>\n \n Radiation and chemical mutagens are direct\n DNA<\/jats:styled-content>\n \u2010damaging agents and ultraviolet (\n UV<\/jats:styled-content>\n ) radiation is frequently used in biological studies. Consequent to ozone depletion,\n UV<\/jats:styled-content>\n \u2010C could become a great challenge to living organisms on earth, in the near future. The present study has focused on the role of poly (\n ADP<\/jats:styled-content>\n \u2010ribose) polymerase (\n PARP<\/jats:styled-content>\n ) during\n UV<\/jats:styled-content>\n \u2010C\u2010induced growth and developmental changes in\n Dictyostelium discoideum,<\/jats:italic>\n a phylogenetically important unicellular eukaryote.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and methods<\/jats:title>\n \n Dictyostelium discoideum<\/jats:italic>\n cells were exposed to different doses of\n UV<\/jats:styled-content>\n \u2010C and\n PARP<\/jats:styled-content>\n activity, and effects of its inhibition were studied. Expression of developmentally regulated genes\n yakA<\/jats:italic>\n ,\n car1<\/jats:italic>\n ,\n aca<\/jats:italic>\n ,\n csA, regA, ctnA, ctnB, gp24<\/jats:italic>\n ,\n hspD<\/jats:italic>\n and\n dsn<\/jats:italic>\n were analysed using semiquantitative\n RT<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n We report that the\n D. discoideum<\/jats:italic>\n cells displayed\n PARP<\/jats:styled-content>\n activation within 2\u00a0min of\n UV<\/jats:styled-content>\n \u2010C irradiation and there was increase in\n NO<\/jats:styled-content>\n levels in a dose\u2010dependent manner.\n UV<\/jats:styled-content>\n \u2010C\u2010irradiated cells had impaired growth, delayed or blocked development and delayed germination compared to control cells. In our previous studies we have shown that inhibition of\n PARP<\/jats:styled-content>\n recovered oxidative stress\u2010induced changes in\n D. discoideum<\/jats:italic>\n ; however, intriguingly\n PARP<\/jats:styled-content>\n inhibition did not correct all defects as effectively in\n UV<\/jats:styled-content>\n \u2010C\u2010irradiated cells. This possibly was due to interplay with increased\n NO<\/jats:styled-content>\n signalling.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Our results signify that\n UV<\/jats:styled-content>\n \u2010C and oxidative stress affected growth and development in\n D. discoideum<\/jats:italic>\n by different mechanisms; these studies could provide major clues to complex mechanisms of growth and development in higher organisms.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/cpr.12182","type":"journal-article","created":{"date-parts":[[2015,4,8]],"date-time":"2015-04-08T08:41:08Z","timestamp":1428482468000},"page":"363-374","source":"Crossref","is-referenced-by-count":12,"title":["Response of\n Dictyostelium discoideum<\/i>\n to\n UV<\/scp>\n \u2010C and involvement of poly (\n ADP<\/scp>\n \u2010ribose) polymerase"],"prefix":"10.1111","volume":"48","author":[{"given":"H.","family":"Mir","sequence":"first","affiliation":[{"name":"Department of Biochemistry Faculty of Science The Maharaja Sayajirao University of Baroda Vadodara Gujarat 390002 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Alex","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Science The Maharaja Sayajirao University of Baroda Vadodara Gujarat 390002 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Rajawat","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Science The Maharaja Sayajirao University of Baroda Vadodara Gujarat 390002 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Kadam","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Science The Maharaja Sayajirao University of Baroda Vadodara Gujarat 390002 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Begum","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Science The Maharaja Sayajirao University of Baroda Vadodara Gujarat 390002 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,8]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"223","article-title":"Signaling molecules involved in the transition of growth to development of Dictyostelium discoideum","volume":"45","author":"Mir HA","year":"2007","journal-title":"Indian J. 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