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Impaired proliferative\/differentiational potentials of adult neural progenitor cells (<jats:styled-content style=\"fixed-case\">ANPC<\/jats:styled-content>s) have been thought to be a pathogenic mechanism involved in it. In this study, we aimed to elucidate intrinsic properties of <jats:styled-content style=\"fixed-case\">ANPC<\/jats:styled-content>s subjected to neurodegenerative condition in <jats:styled-content style=\"fixed-case\">YAC<\/jats:styled-content>128 <jats:styled-content style=\"fixed-case\">HD<\/jats:styled-content> mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">ANPC<\/jats:styled-content>s were isolated from the <jats:styled-content style=\"fixed-case\">SVZ<\/jats:styled-content> regions of 4\u2010month\u2010old <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">YAC<\/jats:styled-content>128 mice. Cell proliferation, migration and neuronal differentiation <jats:italic>in\u00a0vitro<\/jats:italic> were compared between these two genotypes with\/without Ca<jats:sup>2+<\/jats:sup> inhibitors or <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> scavenger treatments. Differences in <jats:styled-content style=\"fixed-case\">ANPC<\/jats:styled-content> proliferation and differentiation capabilities <jats:italic>in\u00a0vivo<\/jats:italic> between the two genotypes were evaluated using Ki\u201067 and Doublecortin (<jats:styled-content style=\"fixed-case\">DCX<\/jats:styled-content>) immunofluorescence respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared to <jats:styled-content style=\"fixed-case\">WT ANPC<\/jats:styled-content>s, <jats:styled-content style=\"fixed-case\">YAC<\/jats:styled-content>128 <jats:styled-content style=\"fixed-case\">ANPC<\/jats:styled-content>s had significantly enhanced cell proliferation, migration and neuronal differentiation <jats:italic>in\u00a0vitro<\/jats:italic>, accompanied by increased Ca<jats:sup>2+<\/jats:sup> and <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> signals. Raised proliferation and migration in <jats:styled-content style=\"fixed-case\">YAC<\/jats:styled-content>128 <jats:styled-content style=\"fixed-case\">ANPC<\/jats:styled-content>s were abolished by Ca<jats:sup>2+<\/jats:sup> signalling antagonists and <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> scavenging. However, <jats:italic>in\u00a0vivo<\/jats:italic>,<jats:styled-content style=\"fixed-case\"> HD ANPC<\/jats:styled-content>s failed to show any elevated proliferation or differentiation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Increased Ca<jats:sup>2+<\/jats:sup> signalling and higher level of <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> conferred <jats:styled-content style=\"fixed-case\">HD ANPC<\/jats:styled-content> enhancement of proliferation and migration potentials. However, the <jats:italic>in\u00a0vivo<\/jats:italic> micro\u2010environment did not support endogenous <jats:styled-content style=\"fixed-case\">ANPC<\/jats:styled-content>s to respond appropriately to neuronal loss in these <jats:styled-content style=\"fixed-case\">YAC<\/jats:styled-content>128 mouse brains.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12205","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T08:58:19Z","timestamp":1439456299000},"page":"517-531","source":"Crossref","is-referenced-by-count":14,"title":["Adult neural progenitor cells from Huntington's disease mouse brain exhibit increased proliferation and migration due to enhanced calcium and <scp>ROS<\/scp> signals"],"prefix":"10.1111","volume":"48","author":[{"given":"Wenjuan","family":"Xie","sequence":"first","affiliation":[{"name":"State Key Laboratory of Biomembrane and Membrane Biotechnology Institute of Zoology Chinese Academy of Sciences Beijing 100101 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