{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T16:38:05Z","timestamp":1757781485756},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,10,12]],"date-time":"2015-10-12T00:00:00Z","timestamp":1444608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Kaneka Corporation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Availability of cord blood (<jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>) processing has been limited by the need for electrically aided centrifugal techniques, which often produce only low final cell product yield. Here, we describe development and characterization of a novel filter device aimed at allowing <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content> processing, using gentle gravity\u2010led flow.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content> was processed with a novel filter device (CellEffic <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>, consisting of non\u2010woven fabric), without any centrifugation. Cells were harvested by flushing the filter with either <jats:styled-content style=\"fixed-case\">HES<\/jats:styled-content> or physiological saline solution (SALINE). Differential cell counts and viability analysis, combined with Fluorescence\u2010Activated Cell Sorting (FACS) (total nucleated cells [TNC], mononuclear cells [MNC], <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45+ <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34+ cells, hematopoietic precursor cells [HPCs]) and clonogenic assay, were employed for analysis of <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content> pre\u2010 and post\u2010processing, and after freeze\/thawing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Processing using the novel filter yielded high quality <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> depletion while maintaining good recovery of <jats:styled-content style=\"fixed-case\">TNC<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> MNC<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> CD<\/jats:styled-content>34+, <jats:styled-content style=\"fixed-case\">HPC<\/jats:styled-content>s and colony forming unit (<jats:styled-content style=\"fixed-case\">CFU<\/jats:styled-content>) output. The filter performed equally well using <jats:styled-content style=\"fixed-case\">HES<\/jats:styled-content> or SALINE. Gravity\u2010led flow provided gentle cell movement and protection of the stem cell compartment. Post\u2010thaw <jats:styled-content style=\"fixed-case\">CFU<\/jats:styled-content> output was maintained particularly, an important indicator for <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content> banking.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Geographical limitations of <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content> transplantation and banking have required a non\u2010electrical, non\u2010centrifugal solution. This novel filter CellEffic <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content> device revealed rapid yet gentle cell processing while maintaining the stem\/progenitor cell compartment required for both haematological and regenerative medicine therapies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12217","type":"journal-article","created":{"date-parts":[[2015,10,13]],"date-time":"2015-10-13T00:11:27Z","timestamp":1444695087000},"page":"671-681","source":"Crossref","is-referenced-by-count":3,"title":["Cord blood processing by a novel filtration system"],"prefix":"10.1111","volume":"48","author":[{"given":"N.","family":"Sato","sequence":"first","affiliation":[{"name":"Medical Devices Division Kaneka Corporation Osaka 530\u20108288 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Fricke","sequence":"additional","affiliation":[{"name":"Kaneka Pharma Europe N.V. 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