{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T18:18:36Z","timestamp":1771957116979,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,10,15]],"date-time":"2015-10-15T00:00:00Z","timestamp":1444867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81400196"],"award-info":[{"award-number":["81400196"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To explore effects of hepatocyte growth factor (<jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content>) combined with insulin\u2010like growth factor 1 (<jats:styled-content style=\"fixed-case\">IGF<\/jats:styled-content>\u20101) on transplanted bone marrow mesenchymal stem cells (<jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s), for treatment of acute myocardial ischaemia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>After ligation of the left anterior descending artery, rabbits were divided into a Control group, a Factors group (<jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content>+<jats:styled-content style=\"fixed-case\">IGF<\/jats:styled-content>\u20101), a <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content> group and a Factors+<jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s group. Allogenous <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s (1 \u00d7 10<jats:sup>7<\/jats:sup>) and\/or control\u2010released microspheres of 2 \u03bcg <jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content>+2 \u03bcg <jats:styled-content style=\"fixed-case\">IGF<\/jats:styled-content>\u20101 were intramyocardially injected into infarcted regions. Apoptosis and differentiation of implanted <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s, histological and morphological results, and cardiac remodelling and function were evaluated at different time points. <jats:italic>In vitro<\/jats:italic>,<jats:styled-content style=\"fixed-case\"> BMSC<\/jats:styled-content>s were exposed to <jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> IGF<\/jats:styled-content>\u20101 and both (50 ng\/ml) and subsequently proliferation, migration, myocardial differentiation and apoptosis induced by hypoxia, were analysed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Four weeks post\u2010operatively, the above indices were significantly improved in Factors+<jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s group compared to the others (<jats:italic>P<\/jats:italic> &lt; 0.01), although Factors and <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s group also showed better results than Control group (<jats:italic>P<\/jats:italic> &lt; 0.05). <jats:italic>In vitro<\/jats:italic>,<jats:styled-content style=\"fixed-case\"> HGF<\/jats:styled-content> promoted <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content> migration and differentiation into cardiomyocytes, but inhibited proliferation (<jats:italic>P<\/jats:italic> &lt; 0.05), while <jats:styled-content style=\"fixed-case\">IGF<\/jats:styled-content>\u20101 increased proliferation and migration, and inhibited apoptosis induced by hypoxia (<jats:italic>P<\/jats:italic> &lt; 0.05), but did not induce myocardial differentiation. Combination of <jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">IGF<\/jats:styled-content>\u20101 significantly promoted <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s capacity for migration, differentiation and lack of apoptosis (<jats:italic>P<\/jats:italic> &lt; 0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Combination of <jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">IGF<\/jats:styled-content>\u20101 activated <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s complementarily, and controlled release of the two factors promoted protective potential of transplanted <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s to repair infarcted myocardium. This suggests a new strategy for cell therapies to overcome acute ischemic myocardial injury.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12219","type":"journal-article","created":{"date-parts":[[2015,10,15]],"date-time":"2015-10-15T11:13:37Z","timestamp":1444907617000},"page":"661-670","source":"Crossref","is-referenced-by-count":41,"title":["<scp>HGF<\/scp> and <scp>IGF<\/scp>\u20101 promote protective effects of allogeneic <scp>BMSC<\/scp> transplantation in rabbit model of acute myocardial infarction"],"prefix":"10.1111","volume":"48","author":[{"given":"Guang\u2010Wei","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery The First Hospital of China Medical University Shenyang 110001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian\u2010Xiang","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery The First Hospital of China Medical University Shenyang 110001 China"},{"name":"The Cardiovascular Research Center of China Medical University Shenyang 110001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Yu","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery The First Hospital of China Medical University Shenyang 110001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue\u2010Jun","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The First Hospital of China Medical University Shenyang 110001 China"},{"name":"Department of Anesthesiology of the First Affiliated Hospital of Dalian Medical University Dalian 116000 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xun","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Radiology The First Hospital of China Medical University Shenyang 110001 China"},{"name":"Key Laboratory of Diagnostic Imaging and Interventional Radiology of Liaoning Province The First Hospital of China Medical University Shenyang 110001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue\u2010Yuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology The First Hospital of China Medical University Shenyang 110001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Bing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Echocardiography The First Hospital of China Medical University Shenyang 110001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Lv","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering Peking Union Medical College Beijing 100010 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery The First Hospital of China Medical University Shenyang 110001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da\u2010Qing","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery The First Hospital of China Medical University Shenyang 110001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery The First Hospital of China Medical University Shenyang 110001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,10,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehl388"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000225952.61196.39"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1391"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.827691"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0095247"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12265-013-9518-4"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2011.05.013"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.08.097"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088794"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2010.02.038"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.15283\/ijsc.2013.6.1.67"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.65"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2010.01070.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvp229"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.12.022"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2015.01.004"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0098"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa060186"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.575118"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/hc0102.101442"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2005-0271"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2004-0176"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-013-1855-8"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.08.139"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.35442"},{"key":"e_1_2_8_27_1","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1097\/00029330-200802020-00011","article-title":"Hepatocyte growth factor combined with insulin like growth factor\u20101 improves expression of GATA\u20104 in mesenchymal stem cells cocultured with cardiomyocytes","volume":"121","author":"Li Z","year":"2008","journal-title":"Chin. Med. J. (Engl)"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2002.36158"}],"container-title":["Cell Proliferation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcpr.12219","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cpr.12219","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T05:53:01Z","timestamp":1696312381000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cpr.12219"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10,15]]},"references-count":27,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1111\/cpr.12219"],"URL":"https:\/\/doi.org\/10.1111\/cpr.12219","archive":["Portico"],"relation":{},"ISSN":["0960-7722","1365-2184"],"issn-type":[{"value":"0960-7722","type":"print"},{"value":"1365-2184","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10,15]]}}}