{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T19:52:31Z","timestamp":1770753151682,"version":"3.50.0"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T00:00:00Z","timestamp":1454457600000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470721"],"award-info":[{"award-number":["81470721"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81321002"],"award-info":[{"award-number":["81321002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31170929"],"award-info":[{"award-number":["31170929"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Physiological oxygen tension plays a critical role in homoeostatic maintenance and development of endochondral bone. Based on the proximity between uncalcified cartilage and subchondral bone, and microchannels that serve as a message delivery network between them, we aimed to explore the influence of low oxygen tension on soluble factor secretion in both chondrocytes and osteoblasts, after co\u2010culture.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Contact co\u2010culture was achieved for morphological observation using red fluorescent protein (<jats:styled-content style=\"fixed-case\">RFP<\/jats:styled-content>)\u2010labelled chondrocytes and green fluorescent protein (<jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>)\u2010labelled osteoblasts, and non\u2010contact co\u2010culture achieved by transwell chambers. This was used to screen genetic variation of growth factors in hypoxia, including respective phenotypic markers, factors involving hypoxia and angiogenesis relationships, bone morphogenetic family proteins, and other general factors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed a significant increase in chondrocyte size following co\u2010culture, in both normoxia and hypoxia, but not of osteoblasts. Expression of Aggrecan in chondrocytes and alkaline phosphatase in osteoblasts was down\u2010regulated under hypoxia following co\u2010culture. Under hypoxia, we found that expression of hypoxia\u2010inducible factor\u20101\u03b1, vascular endothelial growth factor\u2010A\/B, <jats:styled-content style=\"fixed-case\">VE<\/jats:styled-content>\u2010cadherin, bone morphogenetic protein\u20102, and insulin\u2010like growth factor\u20101 in chondrocytes, increased, but <jats:italic><jats:styled-content style=\"fixed-case\">HIF<\/jats:styled-content>\u20101<\/jats:italic>\u03b1, platelet\u2010derived growth factor, <jats:italic><jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>\u20105\/\u20106<\/jats:italic> and fibroblast growth factor\u20101 in osteoblasts, decreased.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results not only indicate the importance of crosstalk between chondrocytes and osteoblasts but also improve our understanding of the mechanisms underlying homoeostatic maintenance of endochondral bone.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12239","type":"journal-article","created":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T16:03:44Z","timestamp":1454515424000},"page":"122-133","source":"Crossref","is-referenced-by-count":19,"title":["Physiological oxygen tension modulates soluble growth factor profile after crosstalk between chondrocytes and osteoblasts"],"prefix":"10.1111","volume":"49","author":[{"given":"Tao","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Fu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuwen","family":"Deng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiyu","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sirong","family":"Shi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Zhong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunfeng","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,2,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.12.2425"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2010.04.606"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2007.06.009"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.91B5.21544"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2003.12.005"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"56","DOI":"10.22203\/eCM.v013a06","article-title":"Oxygen, nitric oxide and articular cartilage","volume":"11","author":"Fermor B","year":"2007","journal-title":"Eur. 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