{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T13:11:06Z","timestamp":1789909866330,"version":"4.0.1"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T00:00:00Z","timestamp":1454457600000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Human amniotic membrane (<jats:styled-content style=\"fixed-case\">HAM<\/jats:styled-content>) has been widely used in soft tissue engineering both in its fresh form and decellularized; its efficiency to aid treatment of burn injuries is well known. On the other hand, it has been reported clinically by several studies that human adipose\u2010derived stem cells (<jats:styled-content style=\"fixed-case\">hADSC<\/jats:styled-content>) are a promising cell source for cell therapy for burns. Recently, we have reported a new technique for decellularization of <jats:styled-content style=\"fixed-case\">HAM<\/jats:styled-content>. In this study, potential of prepared decellularized <jats:styled-content style=\"fixed-case\">HAM<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">dHAM<\/jats:styled-content>) as a viable support for proliferation and delivery of <jats:styled-content style=\"fixed-case\">hADSC<\/jats:styled-content> was investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Amniotic membranes were collected, decellularized and preserved according to the protocol described in our previously published study. <jats:styled-content style=\"fixed-case\">hADSC<\/jats:styled-content> were obtained from the patients undergoing elective liposuction surgery and cells were then seeded on the decellularized membrane for various times. Efficiency of the decellularized membrane as a delivery system for <jats:styled-content style=\"fixed-case\">hADSC<\/jats:styled-content> was investigated by <jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> LDH<\/jats:styled-content> specific activity, <jats:styled-content style=\"fixed-case\">DAPI<\/jats:styled-content> staining and SEM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results showed that <jats:styled-content style=\"fixed-case\">dHAM<\/jats:styled-content> provided a supporting microenvironment for cell growth without producing any cytotoxic effects. In addition, the cells were spread out and actively attached to the <jats:styled-content style=\"fixed-case\">dHAM<\/jats:styled-content> scaffold.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results strongly suggest that <jats:styled-content style=\"fixed-case\">dHAM<\/jats:styled-content>s have considerable potential as 3D cell\u2010carrier scaffolds for delivery of <jats:styled-content style=\"fixed-case\">hADSC<\/jats:styled-content>, in tissue engineering and regenerative medicine applications.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12240","type":"journal-article","created":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T15:35:44Z","timestamp":1454513744000},"page":"115-121","source":"Crossref","is-referenced-by-count":79,"title":["Decellularized human amniotic membrane: how viable is it as a delivery system for human adipose tissue\u2010derived stromal cells?"],"prefix":"10.1111","volume":"49","author":[{"given":"M.","family":"Gholipourmalekabadi","sequence":"first","affiliation":[{"name":"Biotechnology Department School of Advanced Technologies in Medicine Shahid Beheshti University of Medical Sciences Tehran 198396\u20103113 Iran"},{"name":"Cellular and Molecular Biology Research Centre Shahid Beheshti University of Medical Sciences Tehran 198396\u20103113 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Sameni","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Biology Research Centre Shahid Beheshti University of Medical Sciences Tehran 198396\u20103113 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dina","family":"Radenkovic","sequence":"additional","affiliation":[{"name":"University College London (UCL) Medical School London WC1E 6BT UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Mozafari","sequence":"additional","affiliation":[{"name":"Bioengineering Research Group Nanotechnology and Advanced Materials Department MERC Tehran 14155\u20104777 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Mossahebi\u2010Mohammadi","sequence":"additional","affiliation":[{"name":"Department of Hematology and Blood Banking Faculty of Medical Sciences Tarbiat Modares University Tehran 14115\u2010111 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Seifalian","sequence":"additional","affiliation":[{"name":"Centre for Nanotechnology &amp; Regenerative Medicine UCL and Royal Free Hospital London NW3 2QG UK"},{"name":"NanoRegMed Ltd London EC1V 4PW UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,2,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(60)90512-3"},{"key":"e_1_2_6_3_1","first-page":"139","volume-title":"The Scientific Basis of Tissue Transplantation. 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