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Gene variation of growth factors of the two cell types, in both hypoxic and normoxic conditions, were screened using semi\u2010quantitative polymerase chain reaction (PCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Co\u2010culture with ASCs and chondrocytes under hypoxia was shown to successfully induce or enhance ASC to chondrogenic differentiation. To be specific, chondrogenic maker genes: <jats:italic>AGC<\/jats:italic>,<jats:italic> COL II<\/jats:italic> and <jats:italic>SOX9<\/jats:italic> were remarkably enhanced in both ASCs and chondrocytes after crosstalk under low oxygen tension. Subsequently, screening growth factors in ASCs and chondrocytes under hypoxia showed that <jats:italic>HIF\u20101<\/jats:italic>\u03b1, <jats:italic>VEGF\u2010A\/B<\/jats:italic>,<jats:italic> BMP\u20102\/\u20104\/\u20106<\/jats:italic>,<jats:italic> FGF\u20102<\/jats:italic> and <jats:italic>IGF\u20101<\/jats:italic> were significantly increased, but not <jats:italic>TGF\u2010<\/jats:italic>\u03b2<jats:italic>1<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results revealed that both hypoxia and co\u2010culture systems can notably enhance chondrogenesis of ASCs as well as increase proliferation of ASCs and chondrocytes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12259","type":"journal-article","created":{"date-parts":[[2016,4,19]],"date-time":"2016-04-19T05:58:50Z","timestamp":1461045530000},"page":"341-351","source":"Crossref","is-referenced-by-count":44,"title":["Effects of low oxygen tension on gene profile of soluble growth factors in co\u2010cultured adipose\u2010derived stromal cells and chondrocytes"],"prefix":"10.1111","volume":"49","author":[{"given":"Sirong","family":"Shi","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Zhong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiyu","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Fu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoru","family":"Shao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunfeng","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,4,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(70)80078-X"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019104"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1097\/00003086-198804000-00019","article-title":"Subchondral pO2, pCO2, pressure, pH, and lactate in human osteoarthritis of the hip","volume":"229","author":"Kiaer T","year":"1988","journal-title":"Clin. 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