{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:30:41Z","timestamp":1759941041974,"version":"3.41.0"},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,8,3]],"date-time":"2016-08-03T00:00:00Z","timestamp":1470182400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009571","name":"Universidad Nacional de San Luis","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009571","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Melatonin and androgens are involved in the regulation of cell proliferation. However, effects of these hormones on pituitary pars distalis (<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>) of male viscachas is not fully understood. In the present study, we analysed melatonin and gonadal androgens\u2019 effects on proliferating cell nuclear antigen (<jats:styled-content style=\"fixed-case\">PCNA<\/jats:styled-content>) expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Pituitary glands from foetuses, immature individuals, prepubertal individuals and adult viscachas during their reproductive cycle, after melatonin administration and after castration, were used.<jats:styled-content style=\"fixed-case\">PCNA<\/jats:styled-content>\u2010ir cells were detected by immunocytochemistry and morphometrically quantified using image analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Total percentage of<jats:styled-content style=\"fixed-case\">PCNA<\/jats:styled-content>\u2010ir cells varied seasonally in the adult pituitary, with maximum values during the reproductive period and minima during gonadal regression periods. Percentages of<jats:styled-content style=\"fixed-case\">PCNA<\/jats:styled-content>\u2010ir cells increased after melatonin administration, whereas it decreased after castration. Caudal end and ventral regions were the<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>zones which were most affected by seasonal variations and castration.<jats:styled-content style=\"fixed-case\">PCNA<\/jats:styled-content>expression was highest in foetal pituitary from midpregnancy. Numbers of<jats:styled-content style=\"fixed-case\">PCNA<\/jats:styled-content>\u2010ir cells decreased during sexual maturity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results demonstrate the effect of gonadal androgens on cell proliferation during the reproductive period and sexual maturity of these animals. Exogenous melatonin increased<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>cell proliferation in adults. Thus, these hormones seem to be involved in different mechanisms that regulate cell renewal of<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>in this seasonally breeding rodent.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12280","type":"journal-article","created":{"date-parts":[[2016,8,3]],"date-time":"2016-08-03T05:58:07Z","timestamp":1470203887000},"page":"644-653","source":"Crossref","is-referenced-by-count":9,"title":["Effects of melatonin and gonadal androgens on cell proliferation in the pituitary of viscachas (<i>Lagostomus maximus maximus<\/i>)"],"prefix":"10.1111","volume":"49","author":[{"given":"G. J.","family":"Rosales","sequence":"first","affiliation":[{"name":"Laboratorio de Histolog\u00eda \u00c1rea Morfolog\u00eda Departamento de Bioqu\u00edmica y Ciencias Biol\u00f3gicas Facultad de Qu\u00edmica Bioqu\u00edmica y Farmacia Universidad Nacional de San Luis 5700 San Luis Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. I.","family":"Busolini","sequence":"additional","affiliation":[{"name":"Laboratorio de Histolog\u00eda \u00c1rea Morfolog\u00eda Departamento de Bioqu\u00edmica y Ciencias Biol\u00f3gicas Facultad de Qu\u00edmica Bioqu\u00edmica y Farmacia Universidad Nacional de San Luis 5700 San Luis Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. H.","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Laboratorio de Histolog\u00eda \u00c1rea Morfolog\u00eda Departamento de Bioqu\u00edmica y Ciencias Biol\u00f3gicas Facultad de Qu\u00edmica Bioqu\u00edmica y Farmacia Universidad Nacional de San Luis 5700 San Luis Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. P.","family":"Filippa","sequence":"additional","affiliation":[{"name":"Laboratorio de Histolog\u00eda \u00c1rea Morfolog\u00eda Departamento de Bioqu\u00edmica y Ciencias Biol\u00f3gicas Facultad de Qu\u00edmica Bioqu\u00edmica y Farmacia Universidad Nacional de San Luis 5700 San Luis Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,8,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092350111"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.58.103"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-002-0271-8"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1810521"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2006.01459.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1087-0792(98)90020-X"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.2478\/v10042-010-0023-1"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms14022410"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0101403"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2016.03.020"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc1950.44.71"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1000323"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00318674"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004290000140"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004290100161"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0185(20010201)262:2<176::AID-AR1022>3.0.CO;2-I"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1076\/brhm.34.2.193.14488"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.10718"},{"key":"e_1_2_7_20_1","first-page":"128921","article-title":"Effects of photoperiod one epididymal and sperm morphology in a wild rodent, the viscacha (Lagostomus maximus maximus)","volume":"2013","author":"Cruce\u00f1o AM","year":"2012","journal-title":"ISRN Anat"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2005.11.012"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000099626"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.21013"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2012.05.003"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.20671"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD13126"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/168047"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(199809)252:1<8::AID-AR2>3.0.CO;2-T"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.4081\/956"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.20556"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.zool.2010.08.001"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-10-0463"},{"key":"e_1_2_7_33_1","first-page":"5","article-title":"Ecolog\u00eda de la vizcacha (Lagostomus maximus maximus) en el nordeste de la provincia de Entre R\u00edos. Revista de Investigaciones Agr\u00edcolas","volume":"10","author":"Llanos AC","year":"1954","journal-title":"Extra Nueva Serie"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1177\/0748730411420062"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1159\/000433440"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1159\/000023575"},{"key":"e_1_2_7_37_1","doi-asserted-by":"crossref","first-page":"4724","DOI":"10.3760\/cma.j.issn.0366-6999.20131965","article-title":"Elevated levels of mitochonrial respiratory complexes activities and ATP production in 17\u2010\u03b2\u2010estradiol\u2010induced prolactin\u2010secretory tumor cells in male rats are inhibited by melatonin in vivo and in vitro","volume":"126","author":"Wang BQ","year":"2013","journal-title":"Chin Med J"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2047-2927.2014.00207.x"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/538529"},{"key":"e_1_2_7_40_1","first-page":"98","article-title":"Gonadal steroids regulate aromatase P450 expression in the rat pituitary","volume":"15","author":"Carretero J","year":"2011","journal-title":"Eur J Anat"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.22434"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00304582"}],"container-title":["Cell Proliferation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcpr.12280","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcpr.12280","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cpr.12280","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T11:48:48Z","timestamp":1749037728000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cpr.12280"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,3]]},"references-count":41,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["10.1111\/cpr.12280"],"URL":"https:\/\/doi.org\/10.1111\/cpr.12280","archive":["Portico"],"relation":{},"ISSN":["0960-7722","1365-2184"],"issn-type":[{"type":"print","value":"0960-7722"},{"type":"electronic","value":"1365-2184"}],"subject":[],"published":{"date-parts":[[2016,8,3]]}}}