{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T03:38:25Z","timestamp":1783654705233,"version":"3.55.0"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,8,30]],"date-time":"2016-08-30T00:00:00Z","timestamp":1472515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Open Foundation of Shandong Provincial Key Laboratory of Dental Tissue Regeneration","award":["SDKQ201504"],"award-info":[{"award-number":["SDKQ201504"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Tongue squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content>) is the most frequent type of oral malignancy. Increasing evidence has shown that mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s play key roles in many biological processes such as cell development, invasion, proliferation, differentiation, metabolism, apoptosis and migration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> analysis was performed to measure miR\u2010137 expression. <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>\u20108 analysis, cell colony formation, wound\u2010healing analysis and invasion were performed to detect resultant cell functions. The direct target of miR\u2010137 was labelled and measured by luciferase assay and Western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We demonstrated that expression of miR\u2010137 was downregulated in <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> tissues compared to matched normal ones. miR\u2010137 expression was downregulated in <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> lines (<jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content>4, <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">UM<\/jats:styled-content>1 and Cal27) compared to the immortalized <jats:styled-content style=\"fixed-case\">NOK<\/jats:styled-content>16B cell line and normal oral keratinocytes in culture (<jats:styled-content style=\"fixed-case\">NHOK<\/jats:styled-content>). In addition, we have shown that miR\u2010137 expression was epigenetically regulated in <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content>s. Overexpression of miR\u2010137 suppressed <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> proliferation and colony formation. Ectopic expression of miR\u2010137 promoted expression of the epithelial biomarker, E\u2010cadherin, and inhibited the mesenchymal biomarker, N\u2010cadherin, as well as vimentin and Snail expression, indicating that miR\u2010137 suppressed <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> epithelial\u2010mesenchymal transition (<jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content>). We also showed that ectopic expression of miR\u2010137 inhibited <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> invasion and migration. In addition, we identified <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>1 as a direct target gene of miR\u2010137 in <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content>1 cells. <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>1 overexpression rescued inhibitory effects exerted by miR\u2010137 on cell proliferation and <jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results indicate that miR\u2010137 acted as a tumour suppressor in <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> by targeting <jats:styled-content 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