{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T12:58:41Z","timestamp":1776689921170,"version":"3.51.2"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,9,14]],"date-time":"2016-09-14T00:00:00Z","timestamp":1473811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Curcumin (capable of inhibiting angiogenic growth of human umbilical vein endothelial cells [<jats:styled-content style=\"fixed-case\">HUVEC<\/jats:styled-content>s]), can be employed in vitro as a model of pathogenesis of corneal neovascularization (<jats:styled-content style=\"fixed-case\">CRNV<\/jats:styled-content>). The aim of this study was to explore regulatory mechanisms of micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (miR) levels after curcumin treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Expression profiles of miRs in curcumin\u2010treated <jats:styled-content style=\"fixed-case\">HUVEC<\/jats:styled-content>s were investigated by miR microassay. Specific mimics and inhibitors of miR\u20101275 or miR\u20101246 were transfected into <jats:styled-content style=\"fixed-case\">HUVEC<\/jats:styled-content>s. Then, their target genes, vascular endothelial growth factor B (<jats:styled-content style=\"fixed-case\">VEGFB<\/jats:styled-content>) and nuclear transcription factor kappa B acting protein (<jats:styled-content style=\"fixed-case\">NKAP<\/jats:styled-content>) were detected by quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, Western blotting assay or immunofluorescence assay. Cell proliferation and cell cycle parameters were measured with the help of <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>\u20108 assay and flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MiR\u20101275 and miR\u20101246 expression levels were up\u2010regulated by curcumin. Administration of the specific mimics and inhibitors of the two miRs led to significant changes in expression of <jats:styled-content style=\"fixed-case\">VEGFB<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">NKAP<\/jats:styled-content> as well as the indicators related to angiogenesis. Anti\u2010angiogenic effect of curcumin depended on expression patterns of the two miRs in that inhibition of either miR interfered with the effect of curcumin. Furthermore, overexpression of <jats:styled-content style=\"fixed-case\">NKAP<\/jats:styled-content> interrupted effects of curcumin on the cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Collectively, our findings demonstrate that curcumin inhibited <jats:styled-content style=\"fixed-case\">HUVEC<\/jats:styled-content> proliferation by up\u2010regulation of miR\u20101275 and miR\u20101246.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12289","type":"journal-article","created":{"date-parts":[[2016,9,14]],"date-time":"2016-09-14T11:48:48Z","timestamp":1473853728000},"page":"751-762","source":"Crossref","is-referenced-by-count":29,"title":["Curcumin inhibits angiogenesis by up\u2010regulation of micro<scp>RNA<\/scp>\u20101275 and micro<scp>RNA<\/scp>\u20101246: a promising therapy for treatment of corneal neovascularization"],"prefix":"10.1111","volume":"49","author":[{"given":"Yanhui","family":"Bai","sequence":"first","affiliation":[{"name":"Department of 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