{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T04:09:04Z","timestamp":1782014944998,"version":"3.54.5"},"reference-count":48,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,9,19]],"date-time":"2016-09-19T00:00:00Z","timestamp":1474243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470721"],"award-info":[{"award-number":["81470721"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Management of chondral defects has long been a challenge due to poor self\u2010healing capacity of articular cartilage. Many approaches, ranging from symptomatic treatment to structural cartilage regeneration, have obtained very limited satisfactory results. Cartilage tissue engineering, which involves optimized combination of novel scaffolds, cell sources and growth factors, has emerged as a promising strategy for cartilage regeneration and repair. In this study, the aim was to investigate the role of poly(\u03b5\u2010caprolactone)\u2010poly(ethylene glycol)\u2010poly(\u03b5\u2010caprolactone) (<jats:styled-content style=\"fixed-case\">PCL<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCL<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> PCEC<\/jats:styled-content>) <jats:styled-content style=\"fixed-case\">PCEC<\/jats:styled-content> scaffold in cartilage repair.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>First, <jats:styled-content style=\"fixed-case\">PCEC<\/jats:styled-content> film was fabricated, and its characteristics were tested using <jats:styled-content style=\"fixed-case\">SEM<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">AFM<\/jats:styled-content>. Cell (<jats:styled-content style=\"fixed-case\">rASC<\/jats:styled-content> \u2013 rat adipose\u2010derived stem cells, and <jats:styled-content style=\"fixed-case\">mASC<\/jats:styled-content>s \u2013 green fluorescent mouse adipose\u2010derived stem cells) morphologies on <jats:styled-content style=\"fixed-case\">PCEC<\/jats:styled-content> film were observed using <jats:styled-content style=\"fixed-case\">SEM<\/jats:styled-content> and fluorescence microscopy, after cell seeding. Tests of cell viability on <jats:styled-content style=\"fixed-case\">PCEC<\/jats:styled-content> film were conducted using the <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>\u20108 assay. Furthermore, full cartilage defects in rats were created, and <jats:styled-content style=\"fixed-case\">PCEC<\/jats:styled-content> films were implanted, to evaluate their healing effects, over 8\u00a0weeks.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>It was found that <jats:styled-content style=\"fixed-case\">PCEC<\/jats:styled-content> film, as a biomaterial implant, possessed good in vitro properties for cell adhesion, migration and proliferation. Importantly, in the in vivo experiment, <jats:styled-content style=\"fixed-case\">PCEC<\/jats:styled-content> film exhibited desirable healing outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results demonstrated that <jats:styled-content style=\"fixed-case\">PCEC<\/jats:styled-content> film was a good scaffold for cartilage tissue engineering for improving cell proliferation and adhesion and could lead to excellent repair of cartilage defects.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12295","type":"journal-article","created":{"date-parts":[[2016,9,20]],"date-time":"2016-09-20T04:02:41Z","timestamp":1474344161000},"page":"729-739","source":"Crossref","is-referenced-by-count":59,"title":["<scp>PCL<\/scp>\u2010<scp>PEG<\/scp>\u2010<scp>PCL<\/scp> film promotes cartilage regeneration in vivo"],"prefix":"10.1111","volume":"49","author":[{"given":"Na","family":"Fu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu China"},{"name":"School of Stomatology Hospital of Stomatology Tianjin Medical University  Tianjin PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinfeng","family":"Liao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyu","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taoran","family":"Tian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bofeng","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,College of Stomatology Xi'an Jiaotong University  Xi'an Shanxi China"},{"name":"Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases College of Stomatology Xi'an Jiaotong University  Xi'an Shanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunfeng","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,9,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2004.11.006"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0749-8063(96)90179-6"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/03635465980260022701"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-857X(21)00341-0"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0749-8063(86)80012-3"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199410063311401"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100070208"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00021232"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(96)85759-0"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-198567060-00013"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0749-8063(95)90065-9"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/17453679008993062"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0749-8063(05)80428-1"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/boneres.2014.12"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/boneres.2014.2"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8493529"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.2217\/rme.13.28"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2273.2002.00579.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(04)00105-4"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.4248\/IJOS10015"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.612"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1076"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.6270"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm060680j"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-005-4713-8"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1039\/b9nr00162j"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(03)00207-2"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/B9NR00243J"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/b9nr00253g"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(199707)36:1<17::AID-JBM3>3.0.CO;2-O"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.1999.5.421"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(199604)30:4<475::AID-JBM5>3.0.CO;2-M"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(199611)32:3<355::AID-JBM8>3.0.CO;2-R"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.1999.5.35"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(19981205)42:3<396::AID-JBM7>3.0.CO;2-E"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1166\/jbn.2013.1556"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1166\/jbn.2010.1123"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12259"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/boneres.2016.5"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12122"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5RA02016F"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1166\/jbn.2015.2053"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12246"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12239"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12218"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2013.07.017"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00167-012-2329-3"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-012-9450-5"}],"container-title":["Cell Proliferation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcpr.12295","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcpr.12295","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cpr.12295","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T09:16:00Z","timestamp":1696324560000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cpr.12295"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,19]]},"references-count":48,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1111\/cpr.12295"],"URL":"https:\/\/doi.org\/10.1111\/cpr.12295","archive":["Portico"],"relation":{},"ISSN":["0960-7722","1365-2184"],"issn-type":[{"value":"0960-7722","type":"print"},{"value":"1365-2184","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,19]]}}}