{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T06:07:32Z","timestamp":1783145252810,"version":"3.54.6"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,1,16]],"date-time":"2017-01-16T00:00:00Z","timestamp":1484524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81371125"],"award-info":[{"award-number":["81371125"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471803"],"award-info":[{"award-number":["81471803"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004884","name":"Education Department of Sichuan Province","doi-asserted-by":"publisher","award":["10ZB030"],"award-info":[{"award-number":["10ZB030"]}],"id":[{"id":"10.13039\/501100004884","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2017,4]]},"abstract":"Abstract<\/jats:title>Objectives<\/jats:title>Osteoporosis (OP<\/jats:styled-content>) is a systemic disease caused by imbalance between bone resorption and bone formation, commonly resulting from post\u2010menopausal oestrogen deficiency. Although osteogenic differentiation potential of adipose\u2010derived stem cells (ASC<\/jats:styled-content>s) has been demonstrated, the effect of OP on osteogenic differentiation of ASC<\/jats:styled-content>s remains unclear. Here, our work has been designed to compare proliferative capacity and osteogenic differentiation ability of ASC<\/jats:styled-content>s obtained from osteoporotic mice and normal control mice.<\/jats:p><\/jats:sec>Materials and methods<\/jats:title>Twenty 14\u2010week\u2010old female C57BL<\/jats:styled-content>\/6 mice were randomly divided into two groups: one, the ovariectomy (OVX<\/jats:styled-content>) group (n=10), the other being the sham operated (Sham) group (n=10). ASC<\/jats:styled-content>s and OP<\/jats:styled-content>\u2010ASC<\/jats:styled-content>s were obtained from subcutaneous fat of female inguinal sites. Cells were passaged three times prior to subsequent experimentation. The xCELL<\/jats:styled-content>igence system was used to monitor cell adhesion and proliferation. Mineralized nodules of differentiated ASC<\/jats:styled-content>s and OP<\/jats:styled-content>\u2010ASC<\/jats:styled-content>s were analysed using Alizarin red staining after osteogenic induction. Expressions of osteogenic\u2010specific genes including osteopontin (Opn<\/jats:italic>) and runt\u2010related transcription factor 2 (Runx2<\/jats:italic>) were assessed by real\u2010time PCR<\/jats:styled-content> and expression of bone\u2010related proteins was detected by Western blotting.<\/jats:p><\/jats:sec>Results<\/jats:title>Numbers of cells in all groups increased steadily for 6\u00a0days; rate of cell proliferation in the Sham group was found to be higher than in the OVX<\/jats:styled-content> group after 48\u00a0hours. Mineralized bone nodular structures were significantly more concentrated in the Sham group than in the OVX<\/jats:styled-content> group by day 21, and mRNA<\/jats:styled-content> levels of Runx2<\/jats:italic> in the OVX<\/jats:styled-content> group were significantly lower than in the Sham group. Transcript levels of genes coding for Opn<\/jats:italic> showed a similar pattern to those of Runx2<\/jats:italic>. Western blot results indicated that protein expression levels of OPN<\/jats:styled-content> and RUNX<\/jats:styled-content>2 in the OVX<\/jats:styled-content> group were lower than those in the Sham group, at each time point.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>These results indicated that the proliferative capacity and osteogenic potential of ASC<\/jats:styled-content>s were significantly impaired in osteoporotic mice compared to normal controls. However, use of autologous transplantation of modified OP<\/jats:styled-content>\u2010ASC<\/jats:styled-content>s for treatment of OP, or combination of composite scaffolds and modified OP<\/jats:styled-content>\u2010ASC<\/jats:styled-content>s for repair of osteoporotic bone defects, can overcome shortcomings of other methods.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12328","type":"journal-article","created":{"date-parts":[[2017,1,16]],"date-time":"2017-01-16T05:54:08Z","timestamp":1484546048000},"source":"Crossref","is-referenced-by-count":22,"title":["Osteogenic differentiation potential of adipose\u2010derived stem cells from ovariectomized mice"],"prefix":"10.1111","volume":"50","author":[{"given":"Lei","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery The Affiliated Stomatology Hospital of Southwest Medical University Luzhou China"},{"name":"Orofacial Reconstruction and Regeneration Laboratory The Affiliated Stomatology Hospital of Southwest Medical University Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenglong","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery The Affiliated Stomatology Hospital of Southwest Medical University Luzhou China"},{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Li","sequence":"additional","affiliation":[{"name":"Orofacial Reconstruction and Regeneration Laboratory The Affiliated Stomatology Hospital of Southwest Medical University Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomei","family":"Xu","sequence":"additional","affiliation":[{"name":"Orofacial Reconstruction and Regeneration Laboratory The Affiliated Stomatology Hospital of Southwest Medical University Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery The Affiliated Stomatology Hospital of Southwest Medical University Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kui","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery The Affiliated Stomatology Hospital of Southwest Medical University Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxiao","family":"Cai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingang","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery The Affiliated Stomatology Hospital of Southwest Medical University Luzhou China"},{"name":"Orofacial Reconstruction and Regeneration Laboratory The Affiliated Stomatology Hospital of Southwest Medical University Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI27071"},{"key":"e_1_2_7_3_1","first-page":"435","article-title":"Osteoporosis \u2010 a current view of pharmacological prevention and treatment","volume":"7","author":"Das S","year":"2013","journal-title":"Drug Des Devel Ther"},{"key":"e_1_2_7_4_1","first-page":"183","article-title":"Application of health belief model in prevention of osteoporosis in volunteers of Khorramabad City Health Centers","volume":"8","author":"Shojaezadeh DSR","year":"2012","journal-title":"Health Syst Resrarch"},{"key":"e_1_2_7_5_1","first-page":"158","article-title":"Effects of an osteoporosis prevention training program on physical activity\u2010related stages of change and self\u2010efficacy among university students, Shiraz, Iran: a Randomized Clinical Trial","volume":"2","author":"Kaveh MH","year":"2014","journal-title":"J Adv Med Educ Prof."},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/boneres.2015.3"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.06.098"},{"key":"e_1_2_7_8_1","first-page":"1","article-title":"Prevention and management of osteoporosis","volume":"921","author":"World Health Organization","year":"2003","journal-title":"World Health Organ Tech Rep Ser"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12131"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2015.152"},{"key":"e_1_2_7_11_1","first-page":"1301","article-title":"On \u201c2015 Guidelines for Prevention and Treatment of Osteoporosis\u201d. 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