{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T12:47:39Z","timestamp":1774529259212,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,3,20]],"date-time":"2017-03-20T00:00:00Z","timestamp":1489968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Health Science and Technology Development Program Project of Jinan","award":["2011-14"],"award-info":[{"award-number":["2011-14"]}]},{"name":"Science and Technology Development Program Project of Jinan","award":["201401082"],"award-info":[{"award-number":["201401082"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Tongue squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content>) is the most common oral tumours. Micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s play crucial roles in many cell processes including cell viability, development, apoptosis, migration and invasion. The role of miR\u2010802 in the <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> is still unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>The miR\u2010802 expression in <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> tissues and cell lines was determined by quantitative real\u2010time polymerase chain reaction. <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>\u20108 assay was performed to measure the cell viability, while the cell invasion assay was used to determine the cell invasion. Dual\u2010luciferase reporter and western blot were used to confirm the potential target gene of miR\u2010802.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In our study, we demonstrated that miR\u2010802 expression was downregulated in <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> tissues and cell lines. Elevated expression of miR\u2010802 suppressed the <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> cell viability and invasion. Moreover, enforced expression of miR\u2010802 increased the expression of E\u2010cadherin, while suppressed the expression of N\u2010cadherin, Snail and Vimentin in the <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> cell. In addition, we identified the mitogen\u2010activated protein kinase 4 (<jats:styled-content style=\"fixed-case\">MAP<\/jats:styled-content>2K4) as a direct target gene of miR\u2010802 in the <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> cell. We also demonstrated that the expression of <jats:styled-content style=\"fixed-case\">MAP<\/jats:styled-content>2K4 was higher in the <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> tissues than that in the adjacent normal tissues. Furthermore, the expression level of <jats:styled-content style=\"fixed-case\">MAP<\/jats:styled-content>2K4 was inversely associated with the expression of miR\u2010802 in <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> tissues. We also demonstrated that the <jats:styled-content style=\"fixed-case\">MAP<\/jats:styled-content>2K4 expression was upregulated in <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> cell lines. Elevated expression of miR\u2010802 inhibited <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> cell viability and invasion through inhibiting <jats:styled-content style=\"fixed-case\">MAP<\/jats:styled-content>2K4 expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our data revealed that miR\u2010802 played as a tumour suppressor gene and might act as a therapeutic target in <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12336","type":"journal-article","created":{"date-parts":[[2017,3,20]],"date-time":"2017-03-20T13:46:40Z","timestamp":1490017600000},"source":"Crossref","is-referenced-by-count":32,"title":["Micro<scp>RNA<\/scp>\u2010802 plays a tumour suppressive role in tongue squamous cell carcinoma through directly targeting 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9017-1580","authenticated-orcid":false,"given":"Qibao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Endodontics Jinan Stomatological Hospital  Jinan Shandong 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