{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T02:20:26Z","timestamp":1777515626041,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,7,6]],"date-time":"2017-07-06T00:00:00Z","timestamp":1499299200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["80\/5\/2010\/\u2010BMS"],"award-info":[{"award-number":["80\/5\/2010\/\u2010BMS"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2017,8]]},"abstract":"Abstract<\/jats:title>Objectives<\/jats:title>Bone marrow derived endothelial progenitor cells (BM<\/jats:styled-content>\u2010EPC<\/jats:styled-content>s) are increased in chronic liver disease (CLD<\/jats:styled-content>). Their role in hepatic fibrosis and regeneration remains an area of intense studies. We investigated the migration and secretory functions of BM<\/jats:styled-content>\u2010EPC<\/jats:styled-content>s in fibrotic mice liver.<\/jats:p><\/jats:sec>Materials and methods<\/jats:title>Bone marrow cells from C57BL<\/jats:styled-content>6\u2010GFP<\/jats:styled-content> mice were transplanted into the femur of irradiated C57BL<\/jats:styled-content>6 mice, followed by CC<\/jats:styled-content>l4<\/jats:sub> doses for 8 weeks, to develop hepatic fibrosis (n<\/jats:italic>\u00a0=\u00a036). Transplanted C57BL<\/jats:styled-content>6 mice without CC<\/jats:styled-content>l4<\/jats:sub> treatment were used as controls. EPC<\/jats:styled-content>s were analyzed in BM<\/jats:styled-content>, blood and liver by flow cytometry and immunofluorescence. VEGF<\/jats:styled-content> and TGF<\/jats:styled-content>\u2010\u03b2 were analysed in the hepatic stellate cells (HSC<\/jats:styled-content>s) and BM<\/jats:styled-content>\u2010EPC<\/jats:styled-content>s co\u2010cultures using ELISA<\/jats:styled-content>s.<\/jats:p><\/jats:sec>Results<\/jats:title>There was a significant migration of EPC<\/jats:styled-content>s from BM<\/jats:styled-content> to blood and to the liver (P<\/jats:italic>\u00a0\u2264\u00a00.01). Percentage of GFP<\/jats:styled-content>+<\/jats:sup>CD<\/jats:styled-content>31+<\/jats:sup> EPC<\/jats:styled-content>s and collagen proportionate area was substantially increased in the liver at 4th week of CC<\/jats:styled-content>l4<\/jats:sub> dosage compared to the controls (19.8% vs 1.9%, P<\/jats:italic>\u00a0\u2264\u00a00.05). Levels of VEGF<\/jats:styled-content> (533.6\u00a0pg\/ml) and TGF<\/jats:styled-content>\u2010\u03b2 (327.44\u00a0pg\/ml) also increased significantly, when HSC<\/jats:styled-content>s were treated with the EPC<\/jats:styled-content> conditioned medium, as compared to controls (25.66\u00a0pg\/ml and 5.87\u00a0pg\/ml, respectively; P<\/jats:italic>\u00a0\u2264\u00a00.001).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Present findings suggest that BM<\/jats:styled-content>\u2010EPC<\/jats:styled-content>s migrate to the liver during CC<\/jats:styled-content>l4\u2010induced liver injury and contribute to fibrosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12355","type":"journal-article","created":{"date-parts":[[2017,7,6]],"date-time":"2017-07-06T13:04:07Z","timestamp":1499346247000},"source":"Crossref","is-referenced-by-count":11,"title":["Bone marrow endothelial progenitor cells activate hepatic stellate cells and aggravate carbon tetrachloride induced liver fibrosis in mice via paracrine factors"],"prefix":"10.1111","volume":"50","author":[{"given":"Manali","family":"Garg","sequence":"first","affiliation":[{"name":"Institute of Liver and Biliary Sciences Department of Molecular and Cellular Medicine New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Savneet","family":"Kaur","sequence":"additional","affiliation":[{"name":"Gautam Buddha University Greater Noida Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arpita","family":"Banik","sequence":"additional","affiliation":[{"name":"Institute of Liver and Biliary Sciences Department of Molecular and Cellular Medicine New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vikash","family":"Kumar","sequence":"additional","affiliation":[{"name":"National Institute of Immunology New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Archana","family":"Rastogi","sequence":"additional","affiliation":[{"name":"Institute of Liver and Biliary Sciences Department of Pathology New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiv K.","family":"Sarin","sequence":"additional","affiliation":[{"name":"Institute of Liver and Biliary Sciences Department of Hepatology New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asok","family":"Mukhopadhyay","sequence":"additional","affiliation":[{"name":"National Institute of Immunology New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6109-0033","authenticated-orcid":false,"given":"Nirupma","family":"Trehanpati","sequence":"additional","affiliation":[{"name":"Institute of Liver and Biliary Sciences Department of Molecular and Cellular Medicine New Delhi 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