{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:33:26Z","timestamp":1791315206123,"version":"4.3.1"},"reference-count":49,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,8,9]],"date-time":"2017-08-09T00:00:00Z","timestamp":1502236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671031"],"award-info":[{"award-number":["81671031"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470721"],"award-info":[{"award-number":["81470721"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Contemporarily, a highly increasing attention was paid to nanoconstructs, particularly <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> nanostructures possessing precise organization, functional manipulation, biocompatibility and biodegradability. Amongst these <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> nanomaterials, tetrahedral DNA nanostructures (<jats:styled-content style=\"fixed-case\">TDN<\/jats:styled-content>s) are a significantly ideal bionanomaterials with focusing on the property that can be internalized into cytoplasm in the absence of transfection. Therefore, the focus of this study was on investigating the influence of <jats:styled-content style=\"fixed-case\">TDN<\/jats:styled-content>s on the chondrocytes locomotion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Tetrahedral DNA nanostructures was confirmed by 6% polyacrylamide gel electrophoresis (<jats:styled-content style=\"fixed-case\">PAGE<\/jats:styled-content>) and dynamic light scattering (<jats:styled-content style=\"fixed-case\">DLS<\/jats:styled-content>). Subsequently, the effect of <jats:styled-content style=\"fixed-case\">TDN<\/jats:styled-content>s on chondrocyte locomotion was investigated by real\u2010time cell analysis (<jats:styled-content style=\"fixed-case\">RTCA<\/jats:styled-content>) and wound healing assay. The variation of relevant genes and proteins was detected by quantitative polymerase chain reaction (<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>), western blotting and immunofluorescence respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We demonstrated that tetrahedral <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> nanostructures have positive influence on chondrocytes locomotion and promoted the expression of <jats:italic>RhoA<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">ROCK<\/jats:styled-content>2<\/jats:italic> and <jats:italic>vinculin<\/jats:italic>. Additionally, upon exposure to <jats:styled-content style=\"fixed-case\">TDN<\/jats:styled-content>s with the concentration of 250\u00a0nmol\u00a0L<jats:sup>\u22121<\/jats:sup>, the chondrocytes were showed the highest motility via both <jats:styled-content style=\"fixed-case\">RTCA<\/jats:styled-content> and wound healing assay. Meanwhile, the <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and protein expression of RhoA, <jats:styled-content style=\"fixed-case\">ROCK<\/jats:styled-content>2 and vinculin were also significantly enhanced with the same concentration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>It can be concluded that the <jats:styled-content style=\"fixed-case\">TDN<\/jats:styled-content>s with the optimal concentration of 250\u00a0nmol\u00a0L<jats:sup>\u22121<\/jats:sup> could extremely promoted the chondrocytes locomotion through facilitating the expression of <jats:italic>RhoA<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">ROCK<\/jats:styled-content>2<\/jats:italic> and <jats:italic>vinculin<\/jats:italic>. These results seemed to reveal that this special three\u2010dimensional <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> tetrahedral nanostructures may be applied to cartilage repair and treatment in the future.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12368","type":"journal-article","created":{"date-parts":[[2017,8,9]],"date-time":"2017-08-09T13:37:10Z","timestamp":1502285830000},"source":"Crossref","is-referenced-by-count":72,"title":["Modulation of chondrocyte motility by tetrahedral <scp>DNA<\/scp> nanostructures"],"prefix":"10.1111","volume":"50","author":[{"given":"Sirong","family":"Shi","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu 610041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyu","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan 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