{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T14:23:47Z","timestamp":1768314227094,"version":"3.49.0"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,1,17]],"date-time":"2018-01-17T00:00:00Z","timestamp":1516147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005851","name":"Shahid Beheshti University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005851","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2018,4]]},"abstract":"Abstract<\/jats:title>Objective<\/jats:title>Over 5% of the world's population suffers from disabling hearing loss. Stem cell homing in target tissue is an important aspect of cell\u2010based therapy, which its augmentation increases cell therapy efficiency. Deferoxamine (DFO<\/jats:styled-content>) can induce the Akt activation, and phosphorylation status of AKT<\/jats:styled-content> (p\u2010AKT<\/jats:styled-content>) upregulates CXC<\/jats:styled-content> chemokine receptor\u20104 (CXCR<\/jats:styled-content>4) expression. We examined whether DFO<\/jats:styled-content> can enhance mesenchymal stem cells (MSC<\/jats:styled-content>s) homing in noise\u2010induced damaged cochlea by PI<\/jats:styled-content>3K\/AKT<\/jats:styled-content> dependent mechanism.<\/jats:p><\/jats:sec>Materials and Methods<\/jats:title>Mesenchymal stem cells were treated with DFO<\/jats:styled-content>. AKT<\/jats:styled-content>, p\u2010AKT<\/jats:styled-content> protein and hypoxia inducible factor 1\u2010 \u03b1 (HIF<\/jats:styled-content>\u20101\u03b1) and CXCR<\/jats:styled-content>4 gene and protein expression was evaluated by RT<\/jats:styled-content>\u2010 PCR<\/jats:styled-content> and Western blot analysis. For in vivo assay, rats were assigned to control, sham, noise exposure groups without any treatment or receiving normal, DFO<\/jats:styled-content>\u2010treated and DFO<\/jats:styled-content> +LY<\/jats:styled-content>294002 (The PI<\/jats:styled-content>3K inhibitor)\u2010treated MSC<\/jats:styled-content>s. Following chronic exposure to 115 dB<\/jats:styled-content> white noise, MSC<\/jats:styled-content>s were injected into the rat cochlea through the round window. Number of Hoechst\u2010 labelled cells was determined in the endolymph after 24\u00a0hours.<\/jats:p><\/jats:sec>Results<\/jats:title>Deferoxamine increased P\u2010AKT<\/jats:styled-content>, HIF<\/jats:styled-content>\u20101\u03b1 and CXCR<\/jats:styled-content>4 expression in MSC<\/jats:styled-content>s compared to non\u2010treated cells. DFO<\/jats:styled-content> pre\u2010conditioning significantly increased the homing ability of MSC<\/jats:styled-content>s into injured ear compared to normal MSC<\/jats:styled-content>s. These effects of DFO<\/jats:styled-content> were blocked by LY<\/jats:styled-content>294002.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Pre\u2010conditioning of MSC<\/jats:styled-content>s by DFO<\/jats:styled-content> before transplantation can improve stem cell homing in the damaged cochlea through PI<\/jats:styled-content>3K\/AKT<\/jats:styled-content> pathway activation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12434","type":"journal-article","created":{"date-parts":[[2018,1,17]],"date-time":"2018-01-17T05:37:00Z","timestamp":1516167420000},"source":"Crossref","is-referenced-by-count":40,"title":["Deferoxamine promotes mesenchymal stem cell homing in noise\u2010induced injured cochlea through PI<\/scp>3K\/AKT<\/scp> pathway"],"prefix":"10.1111","volume":"51","author":[{"given":"A.A.","family":"Peyvandi","sequence":"first","affiliation":[{"name":"Hearing Disorders Research Center Loghman Hakim Medical Center Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.\u2010A.","family":"Abbaszadeh","sequence":"additional","affiliation":[{"name":"Hearing Disorders Research Center Loghman Hakim Medical Center Shahid Beheshti University of Medical Sciences Tehran Iran"},{"name":"Department of Biology and Anatomical Sciences School of Medicine Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. Ahmady","family":"Roozbahany","sequence":"additional","affiliation":[{"name":"Hearing Disorders Research Center Loghman Hakim Medical Center Shahid Beheshti University of Medical Sciences Tehran Iran"},{"name":"G. Raymond Chang School Ryerson University Toronto Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Pourbakht","sequence":"additional","affiliation":[{"name":"Department of Audiology School of Rehabilitation Sciences Iran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Khoshsirat","sequence":"additional","affiliation":[{"name":"Hearing Disorders Research Center Loghman Hakim Medical Center Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. Haddadzade","family":"Niri","sequence":"additional","affiliation":[{"name":"Department of Audiology School of Rehabilitation Sciences Iran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Peyvandi","sequence":"additional","affiliation":[{"name":"Hearing Disorders Research Center Loghman Hakim Medical Center Shahid Beheshti University of Medical Sciences Tehran Iran"},{"name":"Yale University New Haven CT USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9985-2144","authenticated-orcid":false,"given":"S.","family":"Niknazar","sequence":"additional","affiliation":[{"name":"Hearing Disorders Research Center Loghman Hakim Medical Center Shahid Beheshti University of Medical Sciences Tehran 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