{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T17:45:57Z","timestamp":1790531157670,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,7,30]],"date-time":"2018-07-30T00:00:00Z","timestamp":1532908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570467"],"award-info":[{"award-number":["81570467"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700449"],"award-info":[{"award-number":["81700449"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81701493"],"award-info":[{"award-number":["81701493"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2018,10]]},"abstract":"Abstract<\/jats:title>Objectives<\/jats:title>Emerged evidence demonstrates that long non\u2010coding RNA<\/jats:styled-content>s (lncRNA<\/jats:styled-content>s) may play quintessential regulatory roles in the cellular processes, tumourigenesis and the development of disease. Though focally amplified lncRNA<\/jats:styled-content> on chromosome 1 (FAL<\/jats:styled-content>1) has been identified to have crucial functions in many diseases, its biological mechanism in the development of Hirschsprung's disease (HSCR<\/jats:styled-content>) still remains unknown.<\/jats:p><\/jats:sec>Materials and methods<\/jats:title>The expression levels of FAL<\/jats:styled-content>1 in HSCR<\/jats:styled-content> aganglionic tissues and matched normal specimens were detected by quantitative real\u2010time PCR<\/jats:styled-content> (qRT<\/jats:styled-content>\u2010PCR<\/jats:styled-content>). Cell proliferation and migration were detected by Cell Counting Kit\u20108 (CCK<\/jats:styled-content>\u20108) assay, Ethynyl\u2010deoxyuridine (EdU) assay and transwell assay relatively. Cell cycle and apoptosis were assessed using flow cytometer analysis. Moreover, the novel targets of FAL<\/jats:styled-content>1 were confirmed with the help of bioinformatics analysis and dual\u2010luciferase reporter assay. Western blot assay as well as RNA<\/jats:styled-content> immunoprecipitation (RIP<\/jats:styled-content>) assay was conducted to investigate the potential mechanism.<\/jats:p><\/jats:sec>Results<\/jats:title>FAL<\/jats:styled-content>1 expression was markedly down\u2010regulated in HSCR<\/jats:styled-content> aganglionic tissues and decreased FAL<\/jats:styled-content>1 expression was associated with the diagnosis of HSCR<\/jats:styled-content>. Cell functional analyses indicated that FAL<\/jats:styled-content>1 overexpressing notably promoted cell proliferation and migration, while down\u2010regulation of FAL<\/jats:styled-content>1 suppressed cell proliferation and migration. Additionally, Flow cytometry assay demonstrated that knockdown of FAL<\/jats:styled-content>1 induced markedly cell cycle stalled in the G0\/G1 phase. Furthermore, FAL<\/jats:styled-content>1 could positively regulate AKT<\/jats:styled-content>1 expression by competitively binding to miR\u2010637.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>These results illuminated that FAL<\/jats:styled-content>1 may work as a ceRNA<\/jats:styled-content> to modulate AKT<\/jats:styled-content>1 expression via competitively binding to miR\u2010637 in HSCR<\/jats:styled-content>, suggesting that it may be clinically valuable as a biomarker of HSCR<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12489","type":"journal-article","created":{"date-parts":[[2018,7,31]],"date-time":"2018-07-31T05:54:40Z","timestamp":1533016480000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Long non\u2010coding RNA FAL<\/scp>1 functions as a ceRNA<\/scp> to antagonize the effect of miR\u2010637 on the down\u2010regulation of AKT<\/scp>1 in Hirschsprung's disease"],"prefix":"10.1111","volume":"51","author":[{"given":"Yang","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology School of Public Health Nanjing Medical University Nanjing China"},{"name":"Department of Pediatric Surgery Children's Hospital of Nanjing Medical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingling","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology School of Public Health Nanjing Medical University Nanjing China"},{"name":"Department of Pediatric Surgery Children's Hospital of Nanjing Medical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changgui","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Children's Hospital of Nanjing Medical University Nanjing 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