{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T09:54:30Z","timestamp":1790416470339,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,12,3]],"date-time":"2018-12-03T00:00:00Z","timestamp":1543795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate the functions of miR\u2010223\u20103p and <jats:italic>ITGB3<\/jats:italic> in pulmonary arterial hypertension (PAH).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Microarray analysis was used to detect differentially expressed genes and microRNAs. In in vitro models, the expressions of miR\u2010223\u20103p and <jats:italic>ITGB3<\/jats:italic> were detected by qRT\u2010PCR and Western blot. \u03b1\u2010SMA expression and cell proliferation were analysed by immunofluorescence and MTT assay, respectively. In in vivo models, PAH progressions were determined by measuring the levels of mPAP and RVSP. Lung and myocardial tissues were subjected to HE staining and Masson and Sirius red\u2010saturated carbazotic acid staining to investigate the pathological features.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The microarray analysis revealed that ITGB3 was upregulated, while hsa\u2010miR\u2010223\u20103p was downregulated in PAH. After the induction of hypoxia, miR\u2010223\u20103p was downregulated and <jats:italic>ITGB3<\/jats:italic> was upregulated in PASMCs. Hypoxia induction promoted cell proliferation and inhibited \u03b1\u2010SMA expression in PASMCs. Both the upregulation of miR\u2010223\u20103p and the downregulation of ITGB3 attenuated the aberrant proliferation induced by hypoxia conditions. After approximately 4\u00a0weeks, the mPAP and RVSP levels of rats injected with MCT were decreased by the overexpression of miR\u2010223\u20103p or the silencing of <jats:italic>ITGB3<\/jats:italic>. The staining results revealed that both miR\u2010223\u20103p overexpression and <jats:italic>ITGB3<\/jats:italic> knockdown alleviated the pulmonary vascular remodelling and improved the PAH pathological features of rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>MiR\u2010223\u20103p alleviated the progression of PAH by suppressing the expression of <jats:italic>ITGB3<\/jats:italic>, a finding which provides novel targets for clinical treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12550","type":"journal-article","created":{"date-parts":[[2018,12,3]],"date-time":"2018-12-03T12:00:19Z","timestamp":1543838419000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Role of miR\u2010223\u20103p in pulmonary arterial hypertension <i>via<\/i> targeting <i>ITGB3<\/i> in the ECM 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