{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T20:04:28Z","timestamp":1788984268639,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,3,9]],"date-time":"2019-03-09T00:00:00Z","timestamp":1552089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No. 81602492"],"award-info":[{"award-number":["No. 81602492"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Neutrophils are thought to release neutrophil extracellular traps (NETs) to form in response to exogenous bacteria, viruses and other pathogens. However, the mechanisms underlying NET formation during sterile inflammation are still unclear. In this study, we would like to identify neutrophil extracellular traps formation during sterile inflammation and tissue injury and associated pathways and its mechanism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>We identified different injuries such as chemical\u2010induced and trauma\u2010induced formation of NETs and investigated mechanism of the formation of NETs in vitro and in vivo during the treatment of mtDNA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Here, we find the release of mitochondrial DNA (mtDNA) and oxidized mtDNA in acute peripheral tissue trauma models or other chemically induced lung injury, and moreover, endogenous mtDNA and oxidized mtDNA induce the formation of NETs and sterile inflammation. Oxidized mtDNA is a more potent inducer of NETs. Mitochondrial DNA activates neutrophils via cyclic GMP\u2010AMP synthase (cGAS)\u2010STING and the Toll\u2010like receptor 9 (TLR9) pathways and increases the production of neutrophil elastase and extracellular neutrophil\u2010derived DNA in NETs. Mitochondrial DNA also increases the production of reactive oxygen species (ROS) and expression of the NET\u2010associated proteins Rac 2 and peptidylarginine deiminase 4 (PAD4).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Altogether, these findings highlight that endogenous mitochondrial DNA inducted NETs formation and subsequent sterile inflammation and the mechanism associated with NET formation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cpr.12579","type":"journal-article","created":{"date-parts":[[2019,3,9]],"date-time":"2019-03-09T06:34:52Z","timestamp":1552113292000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":89,"title":["Induction of neutrophil extracellular traps during tissue injury: Involvement of STING and Toll\u2010like receptor 9 pathways"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3962-8295","authenticated-orcid":false,"given":"Li","family":"Liu","sequence":"first","affiliation":[{"name":"Lab of Aging Research and Nanotoxicology, State Key Laboratory of Biotherapy, West China Hospital Sichuan University and Collaborative Innovation Center Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Mao","sequence":"additional","affiliation":[{"name":"Lab of Aging Research and Nanotoxicology, State Key Laboratory of Biotherapy, West China Hospital Sichuan University and Collaborative Innovation Center Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bocheng","family":"Xu","sequence":"additional","affiliation":[{"name":"Lab of Aging Research and Nanotoxicology, State Key Laboratory of Biotherapy, West China Hospital Sichuan University and Collaborative Innovation Center Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangxian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Lab of Aging Research and Nanotoxicology, State Key Laboratory of Biotherapy, West China Hospital Sichuan University and Collaborative Innovation Center Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunju","family":"Fang","sequence":"additional","affiliation":[{"name":"Lab of Aging Research and Nanotoxicology, State Key Laboratory of Biotherapy, West China Hospital Sichuan University and Collaborative Innovation Center Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, West China Second Hospital Sichuan University Chengdu Sichuan 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