{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T13:58:26Z","timestamp":1790431106724,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31601096"],"award-info":[{"award-number":["31601096"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871818"],"award-info":[{"award-number":["81871818"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Low back pain becomes a common orthopaedic disease today. It is mainly induced by the degeneration of the intervertebral disc. In this study, we tried to reveal the pathogenesis of the degeneration and the relative therapeutic strategy, which are still elusive.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>We collected 15 degenerative intervertebral tissues and five healthy donors. Nucleus pulposus and annulus fibrosus cells were subcultured. miR\u2010640 expression was determined by qPCR. Computer analysis and luciferase reporter assay were used to confirm miR\u2010640 target genes. Immunohistochemical and immunocytochemical staining was used to trace the proinflammatory cytokines and key transductor of signalling pathways. We also used \u03b2\u2010galactosidase staining, flow cytometry, and cell viability assay to monitor the degenerative index.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>miR\u2010640 overexpressed in patients derived degenerative nucleus pulposus tissues and cells. The inflammatory environment promoted miR\u2010640 expression via NF\u2010\u03baB signalling pathway. In addition, miR\u2010640 targeted to LRP1 and enhances NF\u2010\u03baB signal activity, which built a positive feedback loop. miR\u2010640 inhibited the expression of \u03b2\u2010catenin and EP300, therefore, restrained WNT signal and induced the degeneration in nucleus pulposus cells. miR\u2010640 inhibitor treatment exhibited the effects of anti\u2010inflammation, reverse WNT signalling pathway exhaustion, and remission of degenerative characteristics in vitro.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>miR\u2010640 plays an important role in the degeneration of intervertebral disc and the relative inflammatory microenvironment. It is a promising potential therapeutic target for the low back pain biotherapy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cpr.12664","type":"journal-article","created":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T05:56:05Z","timestamp":1564034165000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":73,"title":["miR\u2010640 aggravates intervertebral disc degeneration via NF\u2010\u03baB and WNT signalling pathway"],"prefix":"10.1111","volume":"52","author":[{"given":"Wengang","family":"Dong","sequence":"first","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital of Air Force Medical University Xi\u2019an China"},{"name":"Department of Orthopaedics General Hospital of Lanzhou Military Command Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology Disease 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