{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T11:48:47Z","timestamp":1791460127896,"version":"4.3.4"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,21]],"date-time":"2019-11-21T00:00:00Z","timestamp":1574294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010012","name":"National Aerospace Science Foundation of China","doi-asserted-by":"publisher","award":["31671559"],"award-info":[{"award-number":["31671559"]}],"id":[{"id":"10.13039\/501100010012","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004739","name":"Youth Innovation Promotion Association of the Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["CAS2017114"],"award-info":[{"award-number":["CAS2017114"]}],"id":[{"id":"10.13039\/501100004739","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>In humans, non\u2010obstructive azoospermia (NOA) is a major cause of male infertility. However, the aetiology of NOA is largely unknown. Previous studies reported that protein CK2\u03b2 was abundantly and broadly expressed in spermatogenic cells. Here, we investigate whether protein CK2\u03b2 participates in spermatogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>In this study, we separated spermatogenic cells using STA\u2010PUT velocity sedimentation, analysed the expression pattern of protein CK2\u03b2 by immunoblotting, specifically deleted <jats:italic>Ck2\u03b2<\/jats:italic> gene in early\u2010stage spermatogenic cells by crossing <jats:italic>Ck2\u03b2<jats:sup>fl<\/jats:sup><\/jats:italic> mice with <jats:italic>Stra8\u2010Cre<jats:sup>+<\/jats:sup><\/jats:italic> mice and validated the knockout efficiency by quantitative RT\u2010PCR and immunoblotting. The phenotypes of <jats:italic>Ck2\u03b2<jats:sup>fl\/\u0394<\/jats:sup>;SCre<jats:sup>+<\/jats:sup><\/jats:italic> mice were studied by immunohistochemistry and immunofluorescence. The molecular mechanisms of male germ cell development arrest were elucidated by immunoblotting and TUNEL assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Ablation of <jats:italic>Ck2\u03b2<\/jats:italic> gene triggered excessive germ cell apoptosis, germ cell development arrest, azoospermia and male infertility. Inactivation of <jats:italic>Ck2\u03b2<\/jats:italic> gene caused distinctly reduced expression of <jats:italic>Ck2\u03b1<\/jats:italic>\u2032 gene and CK2\u03b1\u2032 protein.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic>Ck2\u03b2<\/jats:italic> is a vital gene for germ cell survival and male fertility in mice.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12726","type":"journal-article","created":{"date-parts":[[2019,11,22]],"date-time":"2019-11-22T06:52:56Z","timestamp":1574405576000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Deletion of <i>Ck2\u03b2<\/i> gene causes germ cell development arrest and azoospermia in male mice"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9884-9675","authenticated-orcid":false,"given":"Qiu\u2010Xia","family":"Liang","sequence":"first","affiliation":[{"name":"Department of Reproductive Medicine 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