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PKJ2016-Y55 and PWZxq2017-03"],"award-info":[{"award-number":["Nos. PKJ2016-Y55 and PWZxq2017-03"]}]},{"name":"SUMHS Seed Foundation Project","award":["No. HMSF-16-21-010"],"award-info":[{"award-number":["No. HMSF-16-21-010"]}]},{"name":"the Projects of National Natural Science Foundation of China","award":["Grant Nos. 81830076, and 81672146"],"award-info":[{"award-number":["Grant Nos. 81830076, and 81672146"]}]},{"name":"the Projects of Nature Science Foundation of Shanghai, China","award":["No. 19ZR1439200"],"award-info":[{"award-number":["No. 19ZR1439200"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>In peripheral neuropathy, the underlying mechanisms of nerve and muscle degeneration include chronic inflammation and oxidative stress in fibrotic tissues. (\u2010)\u2010Epigallocatechin gallate (EGCG) is a major, active component in green tea and may scavenge free radical oxygen and attenuate inflammation. Conservative treatments such as steroid injection only deal with early, asymptomatic, peripheral neuropathy. In contrast, neurolysis and nerve conduit implantation work effectively for treating advanced stages.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>An EGCG\u2010loaded polycaprolactone (PCL) porous scaffold was fabricated using an integrated moulding method. We evaluated proliferative, oxidative and inflammatory activity of rat Schwann cells (RSCs) and rat skeletal muscle cells (RSMCs) cultured on different scaffolds in vitro. In a rat radiation injury model, we assessed the morphological, electrophysiological and functional performance of regenerated sciatic nerves and gastrocnemius muscles, as well as oxidative stress and inflammation state.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>RSCs and RSMCs exhibited higher proliferative, anti\u2010oxidant and anti\u2010inflammatory states in an EGCG\/PCL scaffold. In vivo studies showed improved nerve and muscle recovery in the EGCG\/PCL group, with increased nerve myelination and muscle fibre proliferation and reduced macrophage infiltration, lipid peroxidation, inflammation and oxidative stress indicators.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The EGCG\u2010modified PCL porous nerve scaffold alleviates cellular oxidative stress and repairs peripheral nerve and muscle structure in rats. It attenuates oxidative stress and inflammation in vivo and may provide further insights into peripheral nerve repair in the future.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12730","type":"journal-article","created":{"date-parts":[[2019,11,20]],"date-time":"2019-11-20T08:38:51Z","timestamp":1574239131000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["(\u2010)\u2010Epigallocatechin gallate\u2010loaded polycaprolactone scaffolds fabricated using a 3D integrated moulding method alleviate immune stress and induce neurogenesis"],"prefix":"10.1111","volume":"53","author":[{"given":"Yun","family":"Qian","sequence":"first","affiliation":[{"name":"Department of Orthopedics Shanghai Jiao Tong University Affiliated Sixth People\u2019s Hospital Shanghai China"},{"name":"Shanghai Sixth People\u2019s Hospital East Affiliated to Shanghai University of Medicine &amp; Health Sciences Shanghai 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