{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T21:17:35Z","timestamp":1777583855009,"version":"3.51.4"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,12,30]],"date-time":"2019-12-30T00:00:00Z","timestamp":1577664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2018M640567"],"award-info":[{"award-number":["2018M640567"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81501906"],"award-info":[{"award-number":["81501906"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572124"],"award-info":[{"award-number":["81572124"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81802228"],"award-info":[{"award-number":["81802228"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Insulin\u2010like growth factor\u2010binding protein 7 (IGFBP7) is a low\u2010affinity insulin growth factor (IGF) binder that may play an important role in bone metabolism. We previously reported that IGFBP7 enhanced osteogenic differentiation of bone marrow\u2010derived mesenchymal stem cells (BMSCs) via the Wnt\/\u03b2\u2010catenin signalling pathway. In this study, we tried to reveal its function in osteoclast differentiation and osteoporosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used both in vitro and in vivo studies to investigate the effects of IGFBP7 on RANKL\u2010induced osteoclastogenesis and osteoporosis, together with the underlying molecular mechanisms of these processes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We show that IGFBP7 inhibited receptor activation of nuclear factor\u2010\u03baB (NF\u2010\u03baB) ligand (RANKL)\u2010induced osteoclastogenesis, F\u2010actin ring formation and bone resorption, which was confirmed by using recombinant IGFBP7 protein, lentivirus and siRNA. The NF\u2010\u03baB signalling pathway was inhibited during this process. Moreover, in a mouse ovariectomy\u2010induced osteoporosis model, IGFBP7 treatment attenuated osteoporotic bone loss by inhibiting osteoclast activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Taken together, these findings show that IGFBP7 suppressed osteoclastogenesis in vitro and in vivo and suggest that IGFBP7 is a negative regulator of osteoclastogenesis and plays a protective role in osteoporosis. These novel insights into IGFBP7 may facilitate the development of potential treatment strategies for oestrogen deficiency\u2010induced osteoporosis and other osteoclast\u2010related disorders.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12752","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T04:20:29Z","timestamp":1577766029000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["IGFBP7 acts as a negative regulator of RANKL\u2010induced osteoclastogenesis and oestrogen deficiency\u2010induced bone loss"],"prefix":"10.1111","volume":"53","author":[{"given":"Chenyi","family":"Ye","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou China"},{"name":"Orthopedics Research Institute of Zhejiang University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiduo","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou China"},{"name":"Orthopedics Research Institute of Zhejiang University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mo","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinwei","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou China"},{"name":"Orthopedics Research Institute of Zhejiang University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erman","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou China"},{"name":"Orthopedics Research Institute of Zhejiang University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lan","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou China"},{"name":"Orthopedics Research Institute of Zhejiang University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Hang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou China"},{"name":"Orthopedics Research Institute of Zhejiang University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianhai","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou China"},{"name":"Orthopedics Research Institute of Zhejiang University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou China"},{"name":"Orthopedics Research Institute of Zhejiang University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4858-0684","authenticated-orcid":false,"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou China"},{"name":"Orthopedics Research Institute of Zhejiang University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5937-3878","authenticated-orcid":false,"given":"Rongxin","family":"He","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou China"},{"name":"Orthopedics Research Institute of Zhejiang University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,12,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10677-0"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06898-4"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2014.03.004"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13942"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2018.8361"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26465"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-0540-y"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25907"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/3471215"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.09.125"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13578-019-0310-2"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2018.1470524"},{"key":"e_1_2_10_14_1","first-page":"667","article-title":"Epigenetic downregulation and growth inhibition of IGFBP7 in gastric cancer","volume":"19","author":"Kim J","year":"2018","journal-title":"Asian Pac J Cancer Prev"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-16-2885"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-011-1816-4"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.5.6692"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-014-0105-1"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.4238\/2013.March.13.2"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2004.027342"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0199734"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201700998RR"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-0857-6"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-012-0546-3"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-37091-8"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2015.04.030"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.523811"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.1941"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-011-1697-8"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2771"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-22855-z"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-015-1138-8"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2011.289"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep10227"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/2314769"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2019.03.005"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.12.042"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.39555"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.27288"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401882"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05244-y"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-018-2196-7"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2018.01.005"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2016.05.018"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.3172"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201802364RR"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2536"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.20.6.761"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2012.282"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.060610"}],"container-title":["Cell Proliferation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcpr.12752","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cpr.12752","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cpr.12752","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cpr.12752","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T03:12:56Z","timestamp":1729739576000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cpr.12752"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,30]]},"references-count":49,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["10.1111\/cpr.12752"],"URL":"https:\/\/doi.org\/10.1111\/cpr.12752","archive":["Portico"],"relation":{},"ISSN":["0960-7722","1365-2184"],"issn-type":[{"value":"0960-7722","type":"print"},{"value":"1365-2184","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,30]]},"assertion":[{"value":"2019-09-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12752"}}