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In this study, we aimed to investigate the expression profiling, clinical significance, biological function and the regulation of m6A\u2010related genes in hepatoblastoma (HB).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The mRNA and protein expression levels of m6A\u2010related genes were analysed using Gene Expression Omnibus (GEO) and tissue microarray (TMA) cohort. Kaplan\u2010Meier analysis was performed to evaluate the prognostic value of m6A\u2010related genes in HB. Knockdown of m6A\u2010related genes was conducted to analyse its function on cell proliferation, migration and invasion. Furthermore, bioinformatics analysis and experimental verification were used to explore the potential molecular mechanism and signalling pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that most m6A\u2010related genes were significantly upregulated in HB tumour tissues. High levels of methyltransferase\u2010like 3 (METTL3, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.013), YTHDF2 (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.037) and FTO (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.032) indicated poor clinical outcomes, and the upregulation of METTL3 was an independent prognostic factor in HB patients. Functional assays showed that knockdown of METTL3 could dramatically suppress the proliferation, migration and invasion of HB cells. In addition, METTL3 was identified to be a direct target of microRNA\u2010186 (miR\u2010186). Consistently, miR\u2010186 was low expressed in HB tumour tissues. Moreover, overexpression of miR\u2010186 significantly inhibited cell aggressive phenotype both in vitro and in vivo, while the inhibitory effect could be reversed by METTL3 overexpression. Mechanism study indicated that miR\u2010186\/METTL3 axis contributed to the progression of HB via the Wnt\/\u03b2\u2010catenin signalling pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>M6A\u2010related genes were frequently dysregulated in HB. miR\u2010186\/METTL3\/Wnt\/\u03b2\u2010catenin axis might serve as novel therapeutic targets and prognostic biomarkers in HB.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12768","type":"journal-article","created":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T16:31:49Z","timestamp":1579710709000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":104,"title":["Cross talk between RNA N6\u2010methyladenosine methyltransferase\u2010like 3 and miR\u2010186 regulates hepatoblastoma progression through Wnt\/\u03b2\u2010catenin signalling pathway"],"prefix":"10.1111","volume":"53","author":[{"given":"Xichun","family":"Cui","sequence":"first","affiliation":[{"name":"Pediatric Surgery Department The First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhifang","family":"Wang","sequence":"additional","affiliation":[{"name":"Endocrinology Department The First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhao","family":"Li","sequence":"additional","affiliation":[{"name":"Precision Medicine Center The First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianming","family":"Zhu","sequence":"additional","affiliation":[{"name":"Precision Medicine Center The First Affiliated Hospital of Zhengzhou University Zhengzhou 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