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Morroniside is an iridoid glycoside derived from the Chinese herb <jats:italic>Cornus officinalis<\/jats:italic>, and it has abundant biological activities associated with cell metabolism and tissue regeneration. However, the effects and underlying mechanisms of morroniside on HG\u2010induced BMSC dysfunction remain poorly understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Alkaline phosphatase (ALP) staining, ALP activity and Alizarin Red staining were performed to assess the osteogenesis of BMSCs. Quantitative real\u2010time PCR and Western blot (WB) were used to investigate the osteo\u2010specific markers, receptor for advanced glycation end product (RAGE) signalling and glyoxalase\u20101 (Glo1). Additionally, a T1DM rat model was used to assess the protective effect of morroniside in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Morroniside treatment reverses the HG\u2010impaired osteogenic differentiation of BMSCs in vitro. Morroniside suppressed advanced glycation end product (AGEs) formation and RAGE expression by triggering Glo1. Moreover, the enhanced osteogenesis due to morroniside treatment was partially blocked by the Glo1 inhibitor, BBGCP2. Furthermore, in vivo, morroniside attenuated bone loss and improved bone microarchitecture accompanied by Glo1 upregulation and RAGE downregulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings suggest that morroniside attenuates HG\u2010mediated BMSC dysfunction partly through the inhibition of AGE\u2010RAGE signalling and activation of Glo1 and may be a potential treatment for diabetic osteoporosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12866","type":"journal-article","created":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T07:22:06Z","timestamp":1594279326000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":90,"title":["Morroniside attenuates high glucose\u2013induced BMSC dysfunction by regulating the Glo1\/AGE\/RAGE axis"],"prefix":"10.1111","volume":"53","author":[{"given":"Yi","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery 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