{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T10:28:13Z","timestamp":1784543293381,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T00:00:00Z","timestamp":1603324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81770073"],"award-info":[{"award-number":["81770073"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004204","name":"Tongji University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004204","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009659","name":"Guangzhou Medical University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The high mortality of severe 2019 novel coronavirus disease (COVID\u201019) cases is mainly caused by acute respiratory distress syndrome (ARDS), which is characterized by increased permeability of the alveolar epithelial barriers, pulmonary oedema and consequently inflammatory tissue damage. Some but not all patients showed full functional recovery after the devastating lung damage, and so far there is little knowledge about the lung repair process. We focused on crucial roles of lung progenitor cells in alveolar cell regeneration and epithelial barrier re\u2010establishment and aimed to uncover a possible mechanism of lung repair after severe SARS\u2010CoV\u20102 infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Bronchoalveolar lavage fluid (BALF) of COVID\u201019 patients was analysed by single\u2010cell RNA\u2010sequencing (scRNA\u2010seq). Transplantation of a single KRT5+ cell\u2010derived cell population into damaged mouse lung and time\u2010course scRNA\u2010seq analysis was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In severe (or critical) COVID\u201019 patients, there is a remarkable expansion of TM4SF1+ and KRT5+ lung progenitor cells. The two distinct populations of progenitor cells could play crucial roles in alveolar cell regeneration and epithelial barrier re\u2010establishment, respectively. The transplanted KRT5+ progenitors could long\u2010term engraft into host lung and differentiate into HOPX+ OCLN+ alveolar barrier cell which restored the epithelial barrier and efficiently prevented inflammatory cell infiltration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This work uncovered the mechanism by which various lung progenitor cells work in concert to prevent and replenish alveoli loss post\u2010severe SARS\u2010CoV\u20102 infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.12931","type":"journal-article","created":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T01:46:43Z","timestamp":1603417603000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Single\u2010cell analysis identified lung progenitor cells in COVID\u201019 patients"],"prefix":"10.1111","volume":"53","author":[{"given":"Zixian","family":"Zhao","sequence":"first","affiliation":[{"name":"Shanghai East Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Shanghai East Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueqing","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shanghai East Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofan","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai East Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Regend Therapeutics  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4460-0337","authenticated-orcid":false,"given":"Wei","family":"Zuo","sequence":"additional","affiliation":[{"name":"Shanghai East Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01217-2020"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-020-0369-7"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-020-0536-x"},{"issue":"6","key":"e_1_2_9_5_1","first-page":"568","article-title":"[Pathological changes of fatal coronavirus disease 2019 (COVID\u201019) in the lungs: report of 10 cases by postmortem needle autopsy]","volume":"49","author":"Wu JH","year":"2020","journal-title":"Zhonghua bing li xue za 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