{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T20:55:25Z","timestamp":1754600125168,"version":"3.41.2"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T00:00:00Z","timestamp":1623196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31870973","81871127"],"award-info":[{"award-number":["31870973","81871127"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFC1105102"],"award-info":[{"award-number":["2018YFC1105102"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The loss of LOXL1 expression reportedly leads to the prolapse of pelvic organs or to exfoliation syndrome glaucoma. Increasing evidence suggests that <jats:italic>LOXL1<\/jats:italic> deficiency is associated with the pathogenesis of several other diseases. However, the characterization of the systemic functions of LOXL1 is limited by the lack of relevant investigative technologies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>To determine the functions of LOXL1, a novel method for body\u2010wide organ transcriptome profiling, combined with single\u2010cell mass cytometry, was developed. A body\u2010wide organ transcriptomic (BOT) map was created by RNA\u2010Seq of tissues from 17 organs from both <jats:italic>Loxl1<\/jats:italic> knockout (KO) and wild\u2010type mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The BOT results indicated the systemic upregulation of genes encoding proteins associated with the immune response and proliferation processes in multiple tissues of KO mice, and histological and immune staining confirmed the hyperplasia and infiltration of local immune cells in the tissues of KO mice. Furthermore, mass cytometry analysis of peripheral blood samples revealed systemic immune changes in KO mice. These findings were well correlated with results obtained from cancer databases. Patients with tumours had higher <jats:italic>Loxl1<\/jats:italic> mutation frequencies, and patients with <jats:italic>Loxl1<\/jats:italic>\u2010mutant tumours showed the upregulation of immune processes and cell proliferation and lower survival rates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study provides an effective strategy for the screening of gene functions in multiple organs and also illustrates the important biological roles of LOXL1 in the cells of multiple organs as well as in systemic immunity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.13077","type":"journal-article","created":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T14:30:12Z","timestamp":1623249012000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Mass cytometry and transcriptomic profiling reveal body\u2010wide pathology induced by <i>Loxl1<\/i> deficiency"],"prefix":"10.1111","volume":"54","author":[{"given":"Yu","family":"Li","sequence":"first","affiliation":[{"name":"Clinical Research Center The First Affiliated Hospital School of Medicine Zhejiang University Hangzhou China"},{"name":"Dr. Li Dak Sum &amp; Yip Yio Chin Center for Stem Cell and Regeneration Medicine Zhejiang University Hangzhou China"},{"name":"Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingbing","family":"Wu","sequence":"additional","affiliation":[{"name":"Clinical Research Center The First Affiliated Hospital School of Medicine Zhejiang University Hangzhou China"},{"name":"Dr. Li Dak Sum &amp; Yip Yio Chin Center for Stem Cell and Regeneration Medicine Zhejiang University Hangzhou China"},{"name":"Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine Hangzhou 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