{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T05:23:42Z","timestamp":1761110622462,"version":"3.41.2"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31621004 to QZ","WL"],"award-info":[{"award-number":["31621004 to QZ","WL"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Derivation and maintenance of pluripotent stem cells (PSCs) generally require optimized and complex culture media, which hinders the derivation of PSCs from various species. Expression of Oct4, Sox2, Klf4, and c\u2010Myc (OSKM) can reprogram somatic cells into induced PSCs (iPSCs), even for species possessing no optimal culture condition. Herein, we explored whether expression of OSKM could induce and maintain pluripotency without PSC\u2010specific growth factors and signaling inhibitors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The culture medium of Tet\u2010On\u2010OSKM\/Oct4\u2010GFP mouse embryonic stem cells (ESCs) was switched from N2B27 with MEK inhibitor, GSK3\u03b2 inhibitor, and leukemia inhibitory factor (LIF) (2iL) to N2B27 with doxycycline. Tet\u2010On\u2010OSKM mouse embryonic fibroblast (MEF) cells were reprogrammed in N2B27 with doxycycline. Cell proliferation was traced. Pluripotency was assessed by expression of ESC marker genes, teratoma, and chimera formation. RNA\u2010Seq was conducted to analyze gene expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Via continuous expression of OSKM, mouse ESCs (OSKM\u2010ESCs) and the resulting iPSCs (OSKM\u2010iPSCs) reprogrammed from MEF cells propagated stably, expressed pluripotency marker genes, and formed three germ layers in teratomas. Transcriptional landscapes of OSKM\u2010iPSCs resembled those of ESCs cultured in 2iL and were more similar to those of ESCs cultured in serum\/LIF. Furthermore, OSKM\u2010iPSCs contributed to germline transmission.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Expression of OSKM could induce and maintain mouse pluripotency without specific culturing factors. Importantly, OSKM\u2010iPSCs could produce gene\u2010modified animals through germline transmission, with potential applications in other species.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.13090","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T04:18:57Z","timestamp":1625199537000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Continuous expression of reprogramming factors induces and maintains mouse pluripotency without specific growth factors and signaling inhibitors"],"prefix":"10.1111","volume":"54","author":[{"given":"Yihuan","family":"Mao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences  Beijing China"},{"name":"Institute for Stem Cell and Regenerative Medicine Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences  Beijing 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