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However, its potential function during female germ cell development has not yet been fully understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>HDAC8\u2010targeting siRNA was microinjected into GV oocytes to deplete HDAC8. PCI\u201034051 was used to inhibit the enzyme activity of HDAC8. Immunostaining, immunoblotting and fluorescence intensity quantification were applied to assess the effects of HDAC8 depletion or inhibition on the oocyte meiotic maturation, spindle\/chromosome structure, \u03b3\u2010tubulin dynamics and acetylation level of \u03b1\u2010tubulin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed that HDAC8 was localized in the nucleus at GV stage and then translocated to the spindle apparatus from GVBD to M II stages in porcine oocytes. Depletion of HDAC8 led to the oocyte meiotic failure by showing the reduced polar body extrusion rate. In addition, depletion of HDAC8 resulted in aberrant spindle morphologies and misaligned chromosomes due to the defective recruitment of \u03b3\u2010tubulin to the spindle poles. Notably, these meiotic defects were photocopied by inhibition of HDAC8 activity using its specific inhibitor PCI\u201034051. However, inhibition of HDAC8 did not affect microtubule stability as assessed by the acetylation level of \u03b1\u2010tubulin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Collectively, our findings demonstrate that HDAC8 acts as a regulator of spindle assembly during porcine oocyte meiotic maturation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.13119","type":"journal-article","created":{"date-parts":[[2021,8,26]],"date-time":"2021-08-26T02:19:22Z","timestamp":1629944362000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["HDAC8 drives spindle organization during meiotic maturation of porcine oocytes"],"prefix":"10.1111","volume":"54","author":[{"given":"Ying","family":"Chen","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Nanjing Agricultural University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro\u2010Bioresources Guangxi University Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajuan","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Nanjing Agricultural University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yilong","family":"Miao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Nanjing Agricultural University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2456-4559","authenticated-orcid":false,"given":"Bo","family":"Xiong","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Nanjing Agricultural University 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