{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T16:23:51Z","timestamp":1779121431184,"version":"3.51.4"},"reference-count":37,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,9,25]],"date-time":"2021-09-25T00:00:00Z","timestamp":1632528000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31900690"],"award-info":[{"award-number":["31900690"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771224"],"award-info":[{"award-number":["81771224"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Neurodevelopmental diseases are common disorders caused by the disruption of essential neurodevelopmental processes. Recent human exome sequencing and genome\u2010wide association studies have shown that mutations in the subunits of the SWI\/SNF (BAF) complex are risk factors for neurodevelopmental diseases. Clinical studies have found that ARID1A (BAF250a) is the most frequently mutated SWI\/SNF gene and its mutations lead to mental retardation and microcephaly. However, the function of ARID1A in brain development and its underlying mechanisms still remain elusive.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The present study used Cre\/loxP system to generate an <jats:italic>Arid1a<\/jats:italic> conditional knockout mouse line. Cell proliferation, cell apoptosis and cell differentiation of NSPCs were studied by immunofluorescence staining. In addition, RNA\u2010seq and RT\u2010PCR were performed to dissect the molecular mechanisms of <jats:italic>Arid1a<\/jats:italic> underlying cortical neurogenesis. Finally, rescue experiments were conducted to evaluate the effects of <jats:italic>Neurod1<\/jats:italic> or <jats:italic>Fezf2<\/jats:italic> overexpression on the differentiation of NSPCs in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Conditional knockout of <jats:italic>Arid1a<\/jats:italic> reduces cortical thickness in the developing cortex. <jats:italic>Arid1a<\/jats:italic> loss of function inhibits the proliferation of radial glial cells, and increases cell death during late cortical development, and leads to dysregulated expression of genes associated with proliferation and differentiation. Overexpression of <jats:italic>Neurod1<\/jats:italic> or <jats:italic>Fezf2<\/jats:italic> in <jats:italic>Arid1a<\/jats:italic> cKO NSPCs rescues their neural differentiation defect in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study demonstrates for the first time that <jats:italic>Arid1a<\/jats:italic> plays an important role in regulating the proliferation and differentiation of NSPCs during cortical development, and proposes several gene candidates that are worth to understand the pathological mechanisms and to develop novel interventions of neurodevelopment disorders caused by <jats:italic>Arid1a<\/jats:italic> mutations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.13124","type":"journal-article","created":{"date-parts":[[2021,9,25]],"date-time":"2021-09-25T08:46:53Z","timestamp":1632559613000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Arid1a regulates neural stem\/progenitor cell proliferation and differentiation during cortical development"],"prefix":"10.1111","volume":"54","author":[{"given":"Xiao","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing China"},{"name":"Savaid Medical School University of Chinese Academy of Sciences Beijing China"},{"name":"Institute for Stem Cell and Regeneration Chinese Academy of Sciences Beijing China"},{"name":"Beijing Institute for Stem Cell and Regenerative Medicine Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shang\u2010Kun","family":"Dai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing China"},{"name":"Savaid Medical School University of Chinese Academy of Sciences Beijing China"},{"name":"Institute for Stem Cell and Regeneration 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