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The role of cell heterogeneity of maternal decidua in RSA is yet to be illustrated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>A total of 66,078 single cells from decidua samples isolated from patients with RSA and healthy controls were analysed by unbiased single\u2010cell RNA sequencing (scRNA\u2010seq).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our scRNA\u2010seq results revealed that stromal cells are the most abundant cell type in decidua during early pregnancy. RSA samples are accompanied by aberrant decidualization and obviously obstructed communication between stromal cells and other cell types, such as abnormal activation of macrophages and NK cells. In addition, the over\u2010activated TNF superfamily member 12 (TNFSF12, TWEAK) and FASLG in RSA are closely related to stromal cell demise and pregnancy failure.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our research reveals that the cell composition and communications in normal and RSA decidua at early pregnancy and provides insightful information for the pathology of RSA and will pave the way for pregnancy loss prevention.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cpr.13125","type":"journal-article","created":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T09:01:29Z","timestamp":1632214889000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":102,"title":["Single\u2010cell transcriptome analysis reveals defective decidua stromal niche attributes to recurrent spontaneous abortion"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4014-6138","authenticated-orcid":false,"given":"Lili","family":"Du","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Key Laboratory for Major Obstetric Diseases of Guangdong Province The Third Affiliated Hospital of Guangzhou Medical University Guangzhou China"},{"name":"Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes Guangzhou China"},{"name":"Guangdong Engineering and Technology Research Center of Maternal\u2010Fetal Medicine Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The First Affiliated Hospital of Xiamen University Xiamen University Xiamen China"},{"name":"Key Laboratory of Reproductive Health Research Fujian Province University School of Medicine Xiamen University Xiamen 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