{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T02:36:21Z","timestamp":1791858981178,"version":"4.3.4"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,12,27]],"date-time":"2021-12-27T00:00:00Z","timestamp":1640563200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31730089"],"award-info":[{"award-number":["31730089"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31972538"],"award-info":[{"award-number":["31972538"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Implantation failure is a major cause of prenatal mortality. The uterine lumen closure contributes to embryo adhesion to the uterus, but its underlying mechanisms are largely unknown. Our previous study has reported that endometrial fold extension can lead to uterine lumen closure in pigs. The objective of this study was to reveal molecular mechanisms of the uterine lumen closure by characterizing the molecular basis of the endometrial fold extension during implantation in pigs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Uterine and endometrium tissues during implantation were collected in pigs. MALDI\u2010TOF MS was used to characterize the N\u2010glycomic profiles. Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed that uterine luminal epithelium (LE) migrated collectively during endometrial fold extension. For the first time, we identified a large number of N\u2010glycan compositions from endometrium during implantation using MALDI\u2010TOF MS. Notably, the \u03b12,6\u2010linked sialic acid and ST6GAL1 were highly expressed in uterine LE when the endometrial folds extended greatly. Subsequently, the role of ST6GAL1\u2010mediated 2,6\u2010sialylation in collective epithelial migration was demonstrated. Finally, we found that ST6GAL1\u2010mediated \u03b12,6\u2010sialylation of E\u2010cadherin may participate in collective migration of uterine LE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1\u2010mediated \u03b12,6\u2010sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.13169","type":"journal-article","created":{"date-parts":[[2021,12,27]],"date-time":"2021-12-27T08:46:20Z","timestamp":1640594780000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Glycomics reveal that ST6GAL1\u2010mediated sialylation regulates uterine lumen closure during implantation"],"prefix":"10.1111","volume":"55","author":[{"given":"Kun","family":"Han","sequence":"first","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education College of Animal Science and Technology Huazhong Agricultural University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feiyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education College of Animal Science and Technology Huazhong Agricultural University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulu","family":"Yue","sequence":"additional","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education College of Animal Science and Technology Huazhong Agricultural University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xihong","family":"Tan","sequence":"additional","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding and Reproduction 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