{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T16:02:27Z","timestamp":1778860947955,"version":"3.51.4"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T00:00:00Z","timestamp":1644451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Human brain organoids can provide not only promising models for physiological and pathological neurogenesis but also potential therapies in neurological diseases. However, technical issues such as surgical lesions due to transplantation still limit their applications.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Instead of applying mature organoids, we innovatively developed human brain organoids in vivo by injecting small premature organoids into corpus striatum of adult SCID mice. Two months after injection, single\u2010cell transcriptome analysis was performed on 6131 GFP\u2010labeled human cells from transplanted mouse brains.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Eight subsets of cells (including neuronal cells expressing striatal markers) were identified in these in vivo developed organoids (IVD\u2010organoids) by unbiased clustering. Compared with in vitro cultured human cortical organoids, we found that IVD\u2010organoids developed more supporting cells including pericyte\u2010like and choroid plexus cells, which are important for maintaining organoid homeostasis. Furthermore, IVD\u2010organoids showed lower levels of cellular stress and apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study thus provides a novel method to generate human brain organoids, which is promising in various applications of disease models and therapies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.13201","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T09:44:24Z","timestamp":1644486264000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["In vivo development and single\u2010cell transcriptome profiling of human brain organoids"],"prefix":"10.1111","volume":"55","author":[{"given":"Shichao","family":"Huang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Cell Biology Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences  Shanghai 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Laboratory of Biosystems Homeostasis &amp; Protection and Zhejiang Provincial Key Laboratory for Cancer Molecular Cell Biology Life Sciences Institute Zhejiang University  Hangzhou China"},{"name":"Department of Orthopedics Surgery School of Medicine The Second Affiliated Hospital Zhejiang University  Hangzhou China"},{"name":"Hangzhou Global Scientific and Technological Innovation Center Zhejiang University (HIC\u2010ZJU)  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Pei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cell Biology Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences  Shanghai China"},{"name":"Shanghai Key Laboratory of Signaling and Disease Research Laboratory of Receptor\u2010based Biomedicine The Collaborative Innovation Center for Brain Science School of Life Sciences and Technology Tongji University  Shanghai 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