{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:47:33Z","timestamp":1787014053158,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T00:00:00Z","timestamp":1652832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2019YFA0801402"],"award-info":[{"award-number":["2019YFA0801402"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2014CB964700"],"award-info":[{"award-number":["2014CB964700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31871484"],"award-info":[{"award-number":["31871484"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Early embryo development is dependent on the regulation of maternal messages stored in the oocytes during the maternal\u2010to\u2010zygote transition. Previous studies reported variability of oocyte competence among different inbred mouse strains. The present study aimed to identify the maternal transcripts responsible for early embryonic development by comparing transcriptomes from oocytes of high\u2010 or low\u2010 competence mouse strains.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      <jats:italic>In vitro<\/jats:italic>\n                      fertilization embryos from oocytes of different mouse strains were subject to analysis using microarrays, RNA sequencing, real\u2010time quantitative PCR (RT\u2010qPCR) analysis, Western blotting, and immunofluorescence. One candidate gene,\n                      <jats:italic>Prkce<\/jats:italic>\n                      , was analysed using\n                      <jats:italic>Prkce<\/jats:italic>\n                      knockout mice, followed by a cRNA rescue experiment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The fertilization and 2\u2010cell rate were significantly higher for FVB\/NJ (85.1% and 82.0%) and DBA\/2J (79.6% and 76.7%) inbred mouse strains than those for the MRL\/lpr (39.9% and 35.8%) and 129S3 (35.9% and 36.6%) strains. Thirty\u2010nine differentially expressed genes (DEGs) were noted, of which nine were further verified by RT\u2010qPCR.\n                      <jats:italic>Prkce<\/jats:italic>\n                      knockout mice showed a reduced 2\u2010cell rate (\n                      <jats:italic>Prkce<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>+\/+<\/jats:italic>\n                      <\/jats:sup>\n                      80.1% vs.\n                      <jats:italic>Prkce<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>\u2212\/\u2212<\/jats:italic>\n                      <\/jats:sup>\n                      32.4%) that could be rescued by\n                      <jats:italic>Prkce<\/jats:italic>\n                      cRNA injection (2\u2010cell rate reached 76.7%). Global transcriptional analysis revealed 143 DEGs in the knockout mice, which were largely composed of genes functioning in cell cycle regulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The transcription level of maternal messages such as\n                      <jats:italic>Prkce<\/jats:italic>\n                      in mature oocytes is associated with different 2\u2010cell rates in select inbred mouse strains.\n                      <jats:italic>Prkce<\/jats:italic>\n                      transcript levels could serve as a potential biomarker to characterize high\u2010quality mature oocytes.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cpr.13231","type":"journal-article","created":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T03:44:01Z","timestamp":1652845441000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Maternal Prkce expression in mature oocytes is critical for the first cleavage facilitating maternal\u2010to\u2010zygotic transition in mouse early embryos"],"prefix":"10.1111","volume":"55","author":[{"given":"Shaoqing","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Life Sciences and Biotechnology &amp; Shanghai Children's Hospital Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuli","family":"Gong","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Biotechnology &amp; Shanghai Children's Hospital Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiye","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Biotechnology &amp; Shanghai Children's Hospital Shanghai Jiao Tong University  Shanghai China"},{"name":"Department of Histo\u2010Embryology, Genetics and Developmental Biology Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology &amp; Shanghai Key Laboratory of Embryo and Reproduction Engineering  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingwen","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Biotechnology &amp; 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Shanghai Key Laboratory of Embryo and Reproduction Engineering  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinbing","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Biotechnology &amp; Shanghai Children's Hospital Shanghai Jiao Tong University  Shanghai China"},{"name":"NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology &amp; Shanghai Key Laboratory of Embryo and Reproduction Engineering  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanwen","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Biotechnology &amp; Shanghai Children's Hospital Shanghai Jiao Tong University  Shanghai China"},{"name":"NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology &amp; Shanghai Key Laboratory of Embryo and Reproduction Engineering  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Biotechnology &amp; 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