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Here, we aim to improve the stability of AgNPs by employing a growth anchor graphdiyne (GDY) to overcome these shortcomings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p><jats:italic>Bacillus subtilis<\/jats:italic> and <jats:italic>Escherichia coli<\/jats:italic> were selected to represent gram\u2010positive and gram\u2010negative bacteria, respectively. Transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), scanning electron microscopy (SEM)\u2010EDS mapping and inductively coupled plasma mass spectrometry (ICP\u2010MS) were carried out to characterize the physiochemical properties of materials. The antimicrobial property was determined by turbidimetry and plate colony\u2010counting methods. The physiology of bacteria was detected by SEM and confocal imaging, such as morphology, reactive oxygen species (ROS) and cell membrane.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We successfully synthesized a hybrid graphdiyne @ silver nanoparticles (GDY@Ag) by an environment\u2010friendly approach without any reductants. The hybrid showed high stability and excellent broad\u2010spectrum antibacterial activity towards both gram\u2010positive and gram\u2010negative bacteria. It killed bacteria through membrane destruction and ROS production. Additionally, GDY@Ag did not induce the development of the bacterial resistance after repeated exposure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>GDY@Ag composite combats bacteria by synergetic action of GDY and AgNPs. Especially, GDY@Ag can preserve its bacterial susceptibility after repeated exposure compared to antibiotics. Our findings provide an avenue to design innovative antibacterial agents for effective sterilization.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cpr.13236","type":"journal-article","created":{"date-parts":[[2022,5,3]],"date-time":"2022-05-03T09:01:29Z","timestamp":1651568489000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Insight into the antibacterial resistance of graphdiyne functionalized by silver nanoparticles"],"prefix":"10.1111","volume":"55","author":[{"given":"Simin","family":"Qin","sequence":"first","affiliation":[{"name":"Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology Shanghai Institute of Applied Physics, Chinese Academy of Sciences  Shanghai China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mo","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organic Electronics and Information Displays &amp; Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing University of Posts and Telecommunications  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuting","family":"Cao","sequence":"additional","affiliation":[{"name":"Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology Shanghai Institute of Applied Physics, Chinese Academy of Sciences  Shanghai China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology Shanghai Institute of Applied Physics, Chinese Academy of Sciences  Shanghai China"},{"name":"The Interdisciplinary 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