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Apart from nutritional unsaturated fatty acids, the seeds of peonies are rich in stilbenes characterized by their wide\u2010ranging health\u2010promoting properties. Although the typical stilbene resveratrol has been widely reported for its multiple bioactivities, it remains uncertain whether the trimer of resveratrol trans\u2010gnetin H has properties that regulate cancer cell viability, let alone the underlying mechanism. Autophagy regulated by trans\u2010gnetin H was detected by western blotting, immunofluorescence, and quantitative real\u2010time PCR. The effects of trans\u2010gnetin H on apoptosis and proliferation were examined by flow cytometry, colony formation and Cell Counting Kit\u20108 assays. Trans<jats:italic>\u2010<\/jats:italic>gnetin H significantly inhibits cancer cell viability through autophagy by suppressing the phosphorylation of TFEB and promoting its nuclear transport. Mechanistically, trans\u2010gnetin H inhibits the activation and lysosome translocation of mTORC1 by inhibiting the activation of AMPK, indicating that AMPK is a checkpoint for mTORC1 inactivation induced by trans\u2010gnetin H. Moreover, the binding of TSC2 to Rheb was markedly increased in response to trans\u2010gnetin H stimulation. Similarly, trans\u2010gnetin H inhibited the interaction between Raptor and RagC in an AMPK\u2010dependent manner. More importantly, trans\u2010gnetin H\u2010mediated autophagy highly depends on the AMPK\u2010mTORC1 axis. We propose a regulatory mechanism by which trans\u2010gnetin H inhibits the activation of the mTORC1 pathway to control cell autophagy.<\/jats:p>","DOI":"10.1111\/cpr.13360","type":"journal-article","created":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T10:57:52Z","timestamp":1668509872000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Trans\u2010gnetin H isolated from the seeds of <i>Paeonia<\/i> species induces autophagy via inhibiting mTORC1 signalling through AMPK activation"],"prefix":"10.1111","volume":"56","author":[{"given":"Chao","family":"Xia","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoyan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&amp;F University  Yangling 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