{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T18:56:35Z","timestamp":1793213795516,"version":"4.5.0"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T00:00:00Z","timestamp":1669680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82001111"],"award-info":[{"award-number":["82001111"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["51472115"],"award-info":[{"award-number":["51472115"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20170143"],"award-info":[{"award-number":["BK20170143"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Porphyromonas gingivalis<\/jats:italic> (<jats:italic>P. gingivalis<\/jats:italic>) is a key pathogen of chronic periodontitis. Aryl hydrocarbon receptor (AhR) is essential in immune homeostasis via modulation of pro\u2010inflammatory cytokines production and indoleamine 2,3\u2010dioxygenase (IDO). In this study, it is demonstrated that <jats:italic>P. gingivalis<\/jats:italic> may regulate AhR signalling in periodontitis, which provides a potential target for further immune regulation studies in periodontitis. Experimental periodontitis was induced in C57BL\/6 mice by silk ligature and <jats:italic>P. gingivalis<\/jats:italic> oral inoculation. The alveolar bone resorption was examined using Micro\u2010CT. Histological structures were observed and related cytokines involved in AhR signalling pathway were analysed. RAW264.7 cells were pretreated with AhR agonist (FICZ) and antagonist (CH223191) and infected with <jats:italic>P. gingivalis<\/jats:italic> subsequently. The levels of IDO, AhR and other related cytokines were measured. To demonstrate IDO activity, the concentrations of tryptophan (Trp) and kynurenine (Kyn) were assessed by HPLC. Histological analysis of periodontitis mice showed distinct alveolar bone resorption and inflammatory cell infiltration. The level of AhR and its downstream target factors were significantly decreased in inflamed gingival tissue. Furthermore, RAW 264.7 cells incubated by <jats:italic>P. gingivalis<\/jats:italic> exhibited increased pro\u2010inflammatory cytokines production and decreased AhR, CYP1A1, CYP1B1, and IDO expression. Decreased IDO activity was observed as decreased Kyn\/Trp ratio in the supernatant. Moreover, FICZ decreased the pro\u2010inflammatory cytokines levels in <jats:italic>P. gingivalis<\/jats:italic> infected cells. It is concluded that <jats:italic>P. gingivalis<\/jats:italic> may promote inflammatory responses via inhibiting the AhR signalling pathway in periodontitis.<\/jats:p>","DOI":"10.1111\/cpr.13364","type":"journal-article","created":{"date-parts":[[2022,11,30]],"date-time":"2022-11-30T05:59:57Z","timestamp":1669787997000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["<i>Porphyromonas gingivalis<\/i> infection promotes inflammation via inhibition of the AhR signalling pathway in periodontitis"],"prefix":"10.1111","volume":"56","author":[{"given":"Ting","family":"Hao","sequence":"first","affiliation":[{"name":"Department of Periodontology, Nanjing Stomatological Hospital Medical School of Nanjing University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Nanjing Stomatological Hospital 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