{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T15:14:14Z","timestamp":1784214854348,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,1,2]],"date-time":"2023-01-02T00:00:00Z","timestamp":1672617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102016R1A5A2008630"],"award-info":[{"award-number":["NRF\u20102016R1A5A2008630"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102022R1A2B5B03001627"],"award-info":[{"award-number":["NRF\u20102022R1A2B5B03001627"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Spermatogonial stem cell (SSC) self\u2010renewal is regulated by reciprocal interactions between Sertoli cells and SSCs in the testis. In a previous study, microtubule\u2010associated serine\/threonine kinase 4 (MAST4) has been studied in Sertoli cells as a regulator of SSC self\u2010renewal. The present study focused on the mechanism by which MAST4 in Sertoli cells transmits the signal and regulates SSCs, especially cell cycle regulation. The expression of PLZF, CDK2 and PLZF target genes was examined in WT and <jats:italic>Mast4<\/jats:italic> KO testes by Immunohistochemistry, RT\u2010qPCR and western blot. In addition, IdU and BrdU were injected into WT and <jats:italic>Mast4<\/jats:italic> KO mice and cell cycle of SSCs was analysed. Finally, the testis tissues were cultured in vitro to examine the regulation of cell cycle by MAST4 pathway. <jats:italic>Mast4<\/jats:italic> KO mice showed infertility with Sertoli cell\u2010only syndrome and reduced sperm count. Furthermore, <jats:italic>Mast4<\/jats:italic> deletion led to decreased PLZF expression and cell cycle progression in the testes. MAST4 also induced cyclin\u2010dependent kinase 2 (CDK2) to phosphorylate PLZF and activated PLZF suppressed the transcriptional levels of genes related to cell cycle arrest, leading SSCs to remain stem cell state. MAST4 is essential for maintaining cell cycle in SSCs via the CDK2\u2010PLZF interaction. These results demonstrate the pivotal role of MAST4 regulating cell cycle of SSCs and the significance of spermatogenesis.<\/jats:p>","DOI":"10.1111\/cpr.13390","type":"journal-article","created":{"date-parts":[[2023,1,2]],"date-time":"2023-01-02T23:11:11Z","timestamp":1672701071000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["MAST4 controls cell cycle in spermatogonial stem cells"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3198-157X","authenticated-orcid":false,"given":"Seung\u2010Jun","family":"Lee","sequence":"first","affiliation":[{"name":"Division in Anatomy and Developmental Biology, Department of Oral Biology, Taste Research Center Oral Science Research Center, BK21 FOUR Project, Yonsei University College of Dentistry  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ka\u2010Hwa","family":"Kim","sequence":"additional","affiliation":[{"name":"Division in Anatomy and Developmental Biology, Department of Oral Biology, Taste Research Center Oral Science Research Center, BK21 FOUR Project, Yonsei University College of Dentistry  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6532-9729","authenticated-orcid":false,"given":"Dong\u2010Joon","family":"Lee","sequence":"additional","affiliation":[{"name":"Division in Anatomy and Developmental Biology, Department of Oral Biology, Taste Research Center Oral Science Research Center, BK21 FOUR Project, Yonsei University College of Dentistry  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pyunggang","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of MAST Research Division in GILO Research Institute, GILO Foundation  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinah","family":"Park","sequence":"additional","affiliation":[{"name":"Department of MAST Research Division in GILO Research Institute, GILO Foundation  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong\u2010Jin","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of MAST Research Division in GILO Research Institute, GILO Foundation  Seoul South Korea"},{"name":"Division in Research Institute Laboratory of Musculoskeletal Research, Medpacto Inc.  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